Mouse NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
中文名称：小鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 39.06-2500 pg/mL
- 灵敏度： 23.44 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠NGAL抗体包被于酶标板上，实验时样品（或标准品）中的小鼠NGAL会与包被抗体结合。后依次加入生物素化的抗小鼠NGAL抗体和辣根过氧化物酶标记的亲和素，抗小鼠NGAL抗体与结合在包被抗体上的小鼠NGAL结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NGAL浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NGAL的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 39.06-2500 pg/mL |
| 灵敏度 | 23.44 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠NGAL，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Hierarchical Targeting Nanodrug with Holistic DNA Protection for Effective Treatment of Acute Kidney Injury
IF: 14.300Journal： Advanced Science (2024)Reactivity：MouseSample Type：kidney tissue -
Dynamic changes in the real-time glomerular filtration rate and kidney injury markers in different acute kidney injury models
IF: 6.100Journal： Journal of Translational Medicine (2024)Reactivity：MouseSample Type：kidney tissue -
Unveiling the role of mtDNA in Liver-Kidney Crosstalk: Insights from trichloroethylene hypersensitivity syndrome
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：Serum -
Simiao Pills alleviates renal injury associated with hyperuricemia: A multi-omics analysis
IF: 4.800Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
TNF-α-downregulated SIRT1 regulates mitochondrial-autophagy and apoptosis in renal vascular endothelial cells involved in trichloroethylene-induced immune kidney injury
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
STZ-induced diabetes exacerbates neurons ferroptosis after ischemic stroke by upregulating LCN2 in neutrophils
IF: 4.600 -
Preventive effect of hyperforin on lipopolysaccharide-induced acute kidney injury and inflammation by repressing the NF-κB/miR-21 axis
IF: 1.500Journal： Central European Journal of Immunology (2024)Reactivity：MouseSample Type：Serum -
C5b-9 membrane attack complex activated NLRP3 inflammasome mediates renal tubular immune injury in trichloroethylene sensitized mice
IF: 4.872Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020)PMID：33039874Reactivity：MouseSample Type：Serum,Plasma,Urine -
miR-129-5p alleviates LPS-induced acute kidney injury via targeting HMGB1/TLRs/NF-kappaB pathway
IF: 3.943 -
Trans-10-hydroxy-2-decenoic acid protects against LPS-induced neuroinflammation through FOXO1-mediated activation of autophagy
IF: 4.449Journal： EUROPEAN JOURNAL OF NUTRITION (2019)PMID：31820078Reactivity：MouseSample Type：Cell culture supernatant
实验操作视频
