Mouse NT-proBNP(N-terminal pro-Brain Natriuretic Peptide) ELISA Kit
中文名称：小鼠氨基端前脑钠素(NT-proBNP)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Mouse
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠NT-proBNP抗体包被于酶标板上，实验时样品（或标准品）中的小鼠NT-proBNP会与包被抗体结合。后依次加入生物素化的抗小鼠NT-proBNP抗体和辣根过氧化物酶标记的亲和素，抗小鼠NT-proBNP抗体与结合在包被抗体上的小鼠NT-proBNP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NT-proBNP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NT-proBNP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠NT-proBNP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Circulating mitochondria promoted endothelial cGAS-derived neuroinflammation in subfornical organ to aggravate sympathetic overdrive in heart failure mice
IF: 9.300Empagliflozin protects against heart failure with preserved ejection fraction partly by inhibiting the senescence-associated STAT1–STING axis
IF: 8.500Journal： Cardiovascular Diabetology (2024)Reactivity：MouseSample Type：PlasmaBreviscapine protects against pathological cardiac hypertrophy by targeting FOXO3a-mitofusin-1 mediated mitochondrial fusion
IF: 7.400Development and Evaluation of a Water-Free In Situ Depot Gel Formulation for Long-Acting and Stable Delivery of Peptide Drug ACTY116
IF: 4.900Synergistic effect of collagen peptide LR-7 and taurine on the prevention of cardiovascular injury in high salt-induced hypertensive mice
IF: 4.800Journal： Food Bioscience (2024)Reactivity：MouseSample Type：heart tissueEffects of Phenylacetylglutamine on the Susceptibility of Atrial Fibrillation in Overpressure-Induced HF Mice
IF: 3.200Journal： MOLECULAR AND CELLULAR BIOLOGY (2024)Reactivity：MouseSample Type：PlasmaTrastuzumab-induced cardiomyopathy via ferroptosis-mediated mitochondrial dysfunction
IF: 7.400TNFSF14/LIGHT promotes cardiac fibrosis and atrial fibrillation vulnerability via PI3Kγ/SGK1 pathway-dependent M2 macrophage polarisation
IF: 7.400Axin2 depletion in macrophages alleviated senescence and increased immune response after myocardial infarction
IF: 6.700Astragalus propinquus schischkin and Salvia miltiorrhiza bunge promote angiogenesis to treat myocardial ischemia via Ang-1/Tie-2/FAK pathway
IF: 5.600Journal： Frontiers in Pharmacology (2023)PMID：36699092Reactivity：MouseSample Type：supernatant of blood
