Mouse OC/BGP(Osteocalcin) ELISA Kit
中文名称：小鼠骨钙素(OC/BGP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.39-25 ng/mL
- 灵敏度： 0.23 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠OC/BGP抗体包被于酶标板上，实验时样品（或标准品）中的小鼠OC/BGP会与包被抗体结合。后依次加入生物素化的抗小鼠OC/BGP抗体和辣根过氧化物酶标记的亲和素，抗小鼠OC/BGP抗体与结合在包被抗体上的小鼠OC/BGP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，OC/BGP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中OC/BGP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.39-25 ng/mL |
| 灵敏度 | 0.23 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠OC/BGP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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A Mixture of Cervus elaphus sibiricus and Glycine max (L.) Merrill Inhibits Ovariectomy-Induced Bone Loss Via Regulation of Osteogenic Molecules in a Mouse Model
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID：36902303Reactivity：MouseSample Type：Serum -
Psoralen prevents the inactivation of estradiol and treats osteoporosis via covalently targeting HSD17B2
IF: 5.400Journal： JOURNAL OF ETHNOPHARMACOLOGY (2023)PMID：36997132Reactivity：MouseSample Type：liver tissue -
Drynaria Naringin alleviated mechanical stress deficiency-caused bone loss deterioration via Rspo1/Lgr4-mediated Wnt/β-catenin signalling pathway
IF: 2.100Journal： IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2023)PMID：37831321Reactivity：MouseSample Type：serum -
Aged bone matrix-derived extracellular vesicles as a messenger for calcification paradox
IF: 17.694 -
Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption
IF: 13.417 -
A novel BMP2 secretagogue ameliorates glucocorticoid induced oxidative stress in osteoblasts by activating NRF2 dependent survival while promoting Wnt/β-catenin mediated osteogenesis
IF: 8.101 -
Psoralen suppresses the phosphorylation of amyloid precursor protein (APP) to inhibit myelosuppression
IF: 7.419 -
Activation of farnesoid X receptor signaling by geniposidic acid promotes osteogenesis
IF: 6.656 -
Maternal omega-3 LC-PUFA supplementation programs an improved bone mass in the offspring with a more pronounced effect in females than males at adulthood
IF: 6.117Journal： JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022)PMID：36473540Reactivity：MouseSample Type：plasma -
HDAC inhibitor quisinostat prevents estrogen deficiency-induced bone loss by suppressing bone resorption and promoting bone formation in mice
IF: 5.195
实验操作视频
