Mouse OC/BGP(Osteocalcin) ELISA Kit
中文名称：小鼠骨钙素(OC/BGP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.39-25 ng/mL
- 灵敏度： 0.23 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠OC/BGP抗体包被于酶标板上，实验时样品（或标准品）中的小鼠OC/BGP会与包被抗体结合。后依次加入生物素化的抗小鼠OC/BGP抗体和辣根过氧化物酶标记的亲和素，抗小鼠OC/BGP抗体与结合在包被抗体上的小鼠OC/BGP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，OC/BGP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中OC/BGP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.39-25 ng/mL |
| 灵敏度 | 0.23 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠OC/BGP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Extracellular Vesicles from Child Gut Microbiota Enter into Bone to Preserve Bone Mass and Strength
IF: 16.806 -
Neuronal Induction of Bone-Fat Imbalance through Osteocyte Neuropeptide Y
IF: 16.806 -
Arhgap21 Deficiency Results in Increase of Osteoblastic Lineage Cells in the Murine Bone Marrow Microenvironment
IF: 6.684Journal： Frontiers in Cell and Developmental Biology (2021)Reactivity：Mouse -
Aromatic Bis[aminomethylidenebis(phosphonic)] Acids Prevent Ovariectomy-Induced Bone Loss and Suppress Osteoclastogenesis in Mice
IF: 5.924Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)PMID：34502499Reactivity：MouseSample Type：plasma -
PINK1/Parkin-Mediated Mitophagy Plays a Protective Role in the Bone Impairment Caused by Aluminum Exposure
IF: 5.279Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021)PMID：34018397Reactivity：MouseSample Type：Serum -
Coupling induction of osteogenesis and type H vessels by pulsed electromagnetic fields in ovariectomy-induced osteoporosis in mice
IF: 4.398 -
Deficiency of Omentin-1 leads to delayed fracture healing through excessive inflammation and reduced CD31hiEmcnhi vessels
IF: 4.102Journal： MOLECULAR AND CELLULAR ENDOCRINOLOGY (2021)PMID：34174367Reactivity：MouseSample Type：serum -
Ferulic acid-Cu(II) and Zn(II) complexes promote bone formation
IF: 3.757Journal： PROCESS BIOCHEMISTRY (2021)Reactivity：MouseSample Type：Cell culture supernatant -
Alginate oligosaccharide alleviates senile osteoporosis via the RANKL–RANK pathway in D-galactose-induced C57BL/6J mice
IF: 2.817 -
Protective effects of berberine on senile osteoporosis in mice
IF: 2.626Journal： JOURNAL OF BONE AND MINERAL METABOLISM (2021)PMID：33829324Reactivity：MouseSample Type：Serum
实验操作视频
