Mouse OC/BGP(Osteocalcin) ELISA Kit
中文名称：小鼠骨钙素(OC/BGP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.39-25 ng/mL
- 灵敏度： 0.23 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠OC/BGP抗体包被于酶标板上，实验时样品（或标准品）中的小鼠OC/BGP会与包被抗体结合。后依次加入生物素化的抗小鼠OC/BGP抗体和辣根过氧化物酶标记的亲和素，抗小鼠OC/BGP抗体与结合在包被抗体上的小鼠OC/BGP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，OC/BGP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中OC/BGP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.39-25 ng/mL |
| 灵敏度 | 0.23 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠OC/BGP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3
IF: 9.770 -
CD39 Produced from Human GMSCs Regulates the Balance of Osteoclasts and Osteoblasts through the Wnt/β-Catenin Pathway in Osteoporosis
IF: 8.986 -
Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism
IF: 8.579 -
IL-23, but not IL-12, plays a critical role in inflammation-mediated bone disorders
IF: 8.579 -
Melatonin up-regulates bone marrow mesenchymal stem cells osteogenic action but suppresses their mediated osteoclastogenesis via MT2-inactivated NF-κB pathway
IF: 7.730 -
Akkermansia muciniphila promotes type H vessel formation and bone fracture healing by reducing gut permeability and inflammation
IF: 4.651 -
Inflammatory Skin-Derived Cytokines Accelerate Osteoporosis in Mice with Persistent Skin Inflammation
IF: 4.556Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)PMID：32443893Reactivity：MouseSample Type：serum -
Disordered metabolism in mice lacking irisin
IF: 3.998 -
Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide
IF: 4.959Journal： Materials Science & Engineering C-Materials for Biological Applications (2019)PMID：31499961Reactivity：MouseSample Type：Cell sample -
Blocking glucocorticoid signaling in osteoblasts and osteocytes prevents mechanical unloading-induced cortical bone loss
IF: 4.360
实验操作视频
