Mouse OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit
中文名称:小鼠氧化低密度脂蛋白(OxLDL)酶联免疫吸附测定试剂盒

Price:
- 反应性: Mouse
- 检测范围: 0.31-20 ng/mL
- 灵敏度: 0.19 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠OxLDL抗体包被于酶标板上,实验时样品(或标准品)中的小鼠OxLDL会与包被抗体结合。后依次加入生物素化的抗小鼠OxLDL抗体和辣根过氧化物酶标记的亲和素,抗小鼠OxLDL抗体与结合在包被抗体上的小鼠OxLDL结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,OxLDL浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中OxLDL的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.31-20 ng/mL |
灵敏度 | 0.19 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠OxLDL,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Hypoxia-induced NOS1 as a therapeutic target in hypercholesterolemia-related colorectal cancer
IF: 6.000Journal: Cancer & Metabolism (2024)Reactivity:MouseSample Type:plasma -
Exploring the role of myeloperoxidase in the atherosclerotic process in hypoxic mice based on the MAPK signaling pathway
IF: 5.300Journal: BIOCHEMICAL PHARMACOLOGY (2024)Reactivity:MouseSample Type:plasma,aortic artery homogenates -
Silymarin prevents endothelial dysfunction by upregulating Erk-5 in oxidized LDL exposed endothelial cells
IF: 3.100 -
Bacillus amyloliquefaciens Protect Against Atherosclerosis Through Alleviating Foam Cell Formation and Macrophage Polarization
IF: 2.300Journal: CURRENT MICROBIOLOGY (2024)Reactivity:MouseSample Type:serum -
Burdock fructooligosaccharide ameliorates the hypercholesterolemia and vascular inflammation in mice by regulating cholesterol homeostasis and anti-inflammatory properties
IF: 5.600Journal: Journal of Functional Foods (2023)Reactivity:MouseSample Type:serum -
Choline Supplementation Does Not Promote Atherosclerosis in CETP-Expressing Male Apolipoprotein E Knockout Mice
IF: 6.706 -
Caveolin-1 is a primary determinant of endothelial stiffening associated with dyslipidemia, disturbed flow, and ageing
IF: 4.996 -
Kefir improves blood parameters and reduces cardiovascular risks in patients with metabolic syndrome
-
Chronic treatment with cinnamaldehyde prevents spontaneous atherosclerotic plaque development in ovariectomized LDLr-/- female mice
-
ATP release drives heightened immune responses associated with hypertension
IF: 10.551