Mouse sIgA(Secretory Immunoglobulin A) ELISA Kit
中文名称：小鼠分泌型免疫球蛋白A(sIgA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠sIgA抗体包被于酶标板上，实验时样品（或标准品）中的小鼠sIgA会与包被抗体结合。后依次加入生物素化的抗小鼠sIgA抗体和辣根过氧化物酶标记的亲和素，抗小鼠sIgA抗体与结合在包被抗体上的小鼠sIgA结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，sIgA浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中sIgA的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠sIgA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Aspergillus fumigatus-Derived Extracellular Vesicles Mitigate Fungal Keratitis by Modulating the Immune Cell Function
IF: 5.300 -
DHA-enriched phosphatidylserine ameliorates cyclophosphamide-induced liver injury via regulating the gut-liver axis
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：liver tissue,colon tissue -
Integrative metabolomics and gut microbiota analyses reveal the protective effects of DHA-enriched phosphatidylserine on bisphenol A-induced intestinal damage
IF: 3.800Journal： Journal of Functional Foods (2024)Reactivity：MouseSample Type：jejunum supernatant -
Oral supplementation with Bifidobacterium infantis and 2′-fucosyllactose revives gut microbiota perturbation and intestinal and immune developmental delay following early-life antibiotic challenge in BALB/c mice
IF: 3.700Journal： JOURNAL OF DAIRY SCIENCE (2024)Reactivity：MouseSample Type：cecal tissue -
Comparative analysis of the effects of cyclophosphamide and dexamethasone on intestinal immunity and microbiota in delayed hypersensitivity mice
IF: 2.900Journal： PLoS One (2024)Reactivity：MouseSample Type：Intestinal tissue -
Atractylodes lancea Rhizome Polysaccharide Alleviates Immunosuppression and Intestinal Mucosal Injury in Mice Treated with Cyclophosphamide
IF: 6.100Journal： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2023)PMID：37861444Reactivity：MouseSample Type：intestine -
Bioconversion of feather waste into bioactive nutrients in water by Bacillus licheniformis WHU
IF: 5.000Journal： APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2023)PMID：37750916Reactivity：MouseSample Type：intestine samples -
Bifidobacterium infantis and 2'-Fucosyllactose supplementation in early life may have potential long-term benefits on gut microbiota, intestinal development, and immune function in mice
IF: 3.500 -
Preventive Mechanism of Lycopene on Intestinal Toxicity Caused by Cyclophosphamide Chemotherapy in Mice by Regulating TLR4-MyD88/TRIF-TRAF6 Signaling Pathway and Gut-Liver Axis
IF: 6.706 -
Maternal gestational Bifidobacterium bifidum TMC3115 treatment shapes construction of offspring gut microbiota and development of immune system and induces immune tolerance to food allergen
IF: 6.073Journal： Frontiers in Cellular and Infection Microbiology (2022)Reactivity：MouseSample Type：serum,stomach tissue
Q1:检测小鼠血清里的sIgA，会检测到IgA吗？血清样本中的IgA会干扰sIgA的检测吗？
样本中的IgA是可以检测到的，这主要是基于IgA和sIgA结构的重叠，会存在一定的交叉反应。
小鼠和人类的IgA生物学在几个方面有所不同。在人血清中，IgA主要以单体形式存在，而在小鼠血清中，IgA主要以聚合形式存在。此外，人类IgA可分为密切相关的IgAl和IgA2亚类，其中IgA2较不易被蛋白水解降解。在血清中，IgAl亚类占主导地位，而在分泌物中发现的主要亚型是IgA2，尽管IgAl和IgA2都可以检测到sIgA。
实验操作视频
