Mouse sIgA(Secretory Immunoglobulin A) ELISA Kit
中文名称：小鼠分泌型免疫球蛋白A(sIgA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠sIgA抗体包被于酶标板上，实验时样品（或标准品）中的小鼠sIgA会与包被抗体结合。后依次加入生物素化的抗小鼠sIgA抗体和辣根过氧化物酶标记的亲和素，抗小鼠sIgA抗体与结合在包被抗体上的小鼠sIgA结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，sIgA浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中sIgA的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠sIgA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Early life administration of Bifidobacterium bifidum BD-1 alleviates long-term colitis by remodeling the gut microbiota and promoting intestinal barrier development
IF: 6.064 -
Anti-allergic effects of two potential probiotic strains isolated from infant feces in China
IF: 5.223Journal： Journal of Functional Foods (2022)Reactivity：MouseSample Type：Cecal contents -
Oral S2-Ag85 DNA Vaccine Activated Intestinal Cell dsDNA and RNA Sensors to Promote the Presentation of Intestinal Antigen
IF: 4.493Journal： Journal of Immunology Research (2022)PMID：35465352Reactivity：MouseSample Type：Intestinal segments -
Camelina sativa Oil Treatment Alleviates Castor Oil-Induced Diarrhea in ICR Mice by Regulating Intestinal Flora Composition
IF: 2.650Journal： Evidence-based Complementary and Alternative Medicine (2022)PMID：35178105Reactivity：MouseSample Type：plasma -
Effects of different doses of omega-3 polyunsaturated fatty acids on gut microbiota and immunity
IF: 3.894 -
Emodin Improves Intestinal Health and Immunity through Modulation of Gut Microbiota in Mice Infected by Pathogenic Escherichia coli O1
IF: 2.752 -
Generating Mucosal and Systemic Immune Response Following Vaccination of Vibrio cholerae Adhesion Molecule against Shigella flexneri Infection
IF: 0.985Journal： Indian Journal of Medical Microbiology (2021)PMID：32719207Reactivity：MouseSample Type：ileum mucous -
Betulinic Acid Ameliorates the T-2 Toxin-Triggered Intestinal Impairment in Mice by Inhibiting Inflammation and Mucosal Barrier Dysfunction through the NF-κB Signaling Pathway
IF: 3.531 -
Cyclodextrin/chitosan nanoparticles for oral ovalbumin delivery: Preparation, characterization and intestinal mucosal immunity in mice
IF: 4.560Journal： Asian Journal of Pharmaceutical Sciences (2018)PMID：32104451Reactivity：MouseSample Type：Serum -
Immunopathological assessments of human Blastocystis spp. in experimentally infected immunocompetent and immunosuppresed mice
IF: 2.027Journal： PARASITOLOGY RESEARCH (2016)PMID：26860840Reactivity：MouseSample Type：Serum,Tissue homogenate
实验操作视频
