Mouse TSH(Thyroid Stimulating Hormone) ELISA Kit
中文名称：小鼠促甲状腺素(TSH)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.25-80 ng/mL
- 灵敏度： 0.75 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠TSH抗体包被于酶标板上，实验时样品（或标准品）中的小鼠TSH会与包被抗体结合。后依次加入生物素化的抗小鼠TSH抗体和辣根过氧化物酶标记的亲和素，抗小鼠TSH抗体与结合在包被抗体上的小鼠TSH结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TSH浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TSH的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.25-80 ng/mL |
| 灵敏度 | 0.75 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠TSH，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Repositioning of Cefuroxime as novel selective inhibitor of the thyroid hormone activating enzyme type 2 deiodinase
IF: 9.300 -
Different pharmacokinetics of lithium orotate inform why it is more potent, effective, and less toxic than lithium carbonate in a mouse model of mania
IF: 4.800 -
Arsenic-Induced Thyroid Hormonal Alterations and Their Putative Influence on Ovarian Follicles in Balb/c Mice
IF: 3.900 -
Transcriptome Profile of Thyroid Glands in Bile Duct Ligation Mouse Model
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：35897811Reactivity：MouseSample Type：serum -
Saikosaponin-d Attenuates Hashimoto’s Thyroiditis by Regulating Macrophage Polarization
IF: 4.493 -
TSH Combined with TSHR Aggravates Diabetic Peripheral Neuropathy by Promoting Oxidative Stress and Apoptosis in Schwann Cells
IF: 6.543Journal： Oxidative Medicine and Cellular Longevity (2021)PMID：34804362Reactivity：MouseSample Type：plasma -
Subclinical hypothyroidism contributes to poor glycemic control in patients with type 2 diabetes mellitus, and ellagic acid attenuates methimazole-induced abnormal glucose metabolism in mice model
IF: 2.720 -
The Thyroid Hormone Inactivator Enzyme, Type 3 Deiodinase, Is Essential for Coordination of Keratinocyte Growth and Differentiation
IF: 5.309 -
Renal protective effects of grape seed extract treatment against Eltroxin-induced hyperthyroidism, kidney damage, and oxidative stress in male mice
IF: 3.056Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020)PMID：32166689Reactivity：MouseSample Type：serum -
Chronic exposure to microcystin-LR reduces thyroid hormone levels by activating p38/MAPK and MEK/ERK signal pathway
IF: 4.527Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2019)PMID：30771657Reactivity：MouseSample Type：Plasma
实验操作视频
