Mouse TXB2(Thromboxane B2) ELISA Kit
中文名称:小鼠血栓素B2(TXB2)酶联免疫吸附测定试剂盒

Price:
- 反应性: Mouse
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用小鼠TXB2抗原包被于酶标板上,实验时样品或标准品中的小鼠TXB2与包被的小鼠TXB2竞争生物素标记的抗小鼠TXB2单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,小鼠TXB2浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中小鼠TXB2的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 2 h 30 min |
反应性 | Mouse |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的小鼠TXB2,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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An-Gong-Niu-Huang-Wan (AGNHW) regulates cerebral blood flow by improving hypoperfusion, cerebrovascular reactivity and microcirculation disturbances after stroke
IF: 5.300Journal: Chinese Medicine (2024)Reactivity:MouseSample Type:serum,brain homogenates -
Ginkgolide B alleviates glucolipid metabolism disorders and adipose tissue inflammation by inhibiting thromboxane A2 synthesis
IF: 5.200 -
Targeted elimination of senescent cells by engineered extracellular vesicles attenuates atherosclerosis in ApoE-/- mice with minimal side effects
IF: 12.400 -
Thromboxane A2/Thromboxane A2 receptor axis facilitates hepatic insulin resistance and steatosis through ER stress in NAFLD
IF: 7.300Journal: BRITISH JOURNAL OF PHARMACOLOGY (2023)PMID:37940413Reactivity:MouseSample Type:plasma,liver -
Dual-Driven Hemostats Featured with Puncturing Erythrocytes for Severe Bleeding in Complex Wounds
IF: 11.036 -
Platelet Inhibition by Low-Dose Acetylsalicylic Acid Reduces Neuroinflammation in an Animal Model of Multiple Sclerosis
IF: 5.924Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)PMID:34576080Reactivity:MouseSample Type:plasma -
Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy
IF: 8.063 -
Microthrombotic Renal Vascular Lesions Are Associated to Increased Renal Inflammatory Infiltration in Murine Lupus Nephritis
IF: 5.511 -
Combination Therapy with DETA/NO and Clopidogrel Inhibits Metastasis in Murine Mammary Gland Cancer Models via Improved Vasoprotection
IF: 4.556Journal: MOLECULAR PHARMACEUTICS (2018)PMID:30234992Reactivity:MouseSample Type:Plasma,Tissue homogenate -
Protective effects of marrubiin improve endometriosis through suppression of the expression of RANTES
IF: 1.692Journal: Molecular Medicine Reports (2017)PMID:28713901Reactivity:MouseSample Type:endometriotic cell-Xenograft