Mouse VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit
中文名称：小鼠血管内皮细胞生长因子A(VEGF-A)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠VEGF-A抗体包被于酶标板上，实验时样品（或标准品）中的小鼠VEGF-A会与包被抗体结合。后依次加入生物素化的抗小鼠VEGF-A抗体和辣根过氧化物酶标记的亲和素，抗小鼠VEGF-A抗体与结合在包被抗体上的小鼠VEGF-A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，VEGF-A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中VEGF-A的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠VEGF-A，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa-Ephb2 Pair
IF: 14.300 -
Liquid metal hybrid antibacterial hydrogel scaffolds from 3D printing for wound healing
IF: 13.300 -
ADAM8 deficiency in macrophages promotes cardiac repair after myocardial infarction via ANXA2-mTOR-autophagy pathway
IF: 11.400 -
3D nanofiber scaffolds from 2D electrospun membranes boost cell penetration and positive host response for regenerative medicine
IF: 10.600 -
Legumain-Guided Ferulate-Peptide Self-Assembly Enhances Macrophage-Endotheliocyte Partnership to Promote Therapeutic Angiogenesis After Myocardial Infarction
IF: 10.000 -
Multifunctional SEBS/AgNWs Nanocomposite Films with Antimicrobial, Antioxidant, and Anti-Inflammatory Properties Promote Infected Wound Healing
IF: 8.300Journal： ACS Applied Materials & Interfaces (2024)Reactivity：MouseSample Type：wound tissue -
Unveiling the antitumor synergy between pazopanib and metformin on lung cancer through suppressing p-Akt/ NF-κB/ STAT3/ PD-L1 signal pathway
IF: 6.900Journal： BIOMEDICINE & PHARMACOTHERAPY (2024)Reactivity：MouseSample Type：lung tissue -
Enhancing the Inhibition of Corneal Neovascularization Efficacy by Self-Assembled into Supramolecular Hydrogel of Anti-Angiogenic Peptide
IF: 6.600Journal： International Journal of Nanomedicine (2024)Reactivity：MouseSample Type：corneal tissue -
Evaluation of Vincamine Loaded with Silver Nanoparticles as a New Potential Therapeutic Agent Against Ehrlich’s Solid Carcinoma in Mice
IF: 5.100Journal： Cells (2024)Reactivity：MouseSample Type：Ehrlich solid carcinoma (ESC) tumor cell-Xenograft -
Macrophage M2 polarization promotes pulpal inflammation resolution during orthodontic tooth movement
IF: 4.300
实验操作视频
