Mouse VWF(Von Willebrand Factor) ELISA Kit
中文名称：小鼠血管性血友病因子(VWF)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 1.25-80 ng/mL
- 灵敏度： 0.75 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠VWF抗体包被于酶标板上，实验时样品（或标准品）中的小鼠VWF会与包被抗体结合。后依次加入生物素化的抗小鼠VWF抗体和辣根过氧化物酶标记的亲和素，抗小鼠VWF抗体与结合在包被抗体上的小鼠VWF结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，VWF浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中VWF的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.25-80 ng/mL |
| 灵敏度 | 0.75 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的小鼠VWF，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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A novel role for protein disulfide isomerase ERp18 in venous thrombosis
IF: 2.600Journal： Thrombosis Journal (2024)Reactivity：MouseSample Type：plasma -
Aging Is Associated With Organ-Specific Alterations in the Level and Expression Pattern of von Willebrand Factor
IF: 8.700Journal： ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY (2023)PMID：37732483Reactivity：MouseSample Type：plasma -
Thymosin β4 Exerts a Cytoprotective Function and Attenuates Liver Injury in Murine Hepatic Sinusoidal Obstruction Syndrome after Hematopoietic Stem Cell Transplantation
IF: 3.200Journal： Transplantation and Cellular Therapy (2023)PMID：37192732Reactivity：MouseSample Type：plasma -
Butein ameliorates chronic stress induced atherosclerosis via targeting anti-inflammatory, anti-fibrotic and BDNF pathways
IF: 2.900 -
Platelets are indispensable for alveolar development in neonatal mice
IF: 3.569 -
Acrolein Induces Systemic Coagulopathy via Autophagy-dependent Secretion of von Willebrand Factor in Mice after Traumatic Brain Injury
IF: 5.203 -
Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy
IF: 8.063 -
ADAMTS13 protects mice against renal ischemia-reperfusion injury by reducing inflammation and improving endothelial function
IF: 3.323Journal： AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY (2019)PMID：30461292Reactivity：MouseSample Type：Plasma,Tissue homogenate -
Combination Therapy with DETA/NO and Clopidogrel Inhibits Metastasis in Murine Mammary Gland Cancer Models via Improved Vasoprotection
IF: 4.556Journal： MOLECULAR PHARMACEUTICS (2018)PMID：30234992Reactivity：MouseSample Type：Plasma,Tissue homogenate -
mTOR/STAT-3 pathway mediates mesenchymal stem cell–secreted hepatocyte growth factor protective effects against lipopolysaccharide-induced vascular endothelial barrier dysfunction and apoptosis
IF: 2.959Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2018)PMID：30242894Reactivity：MouseSample Type：Cell culture supernatant
实验操作视频
