NA/NE(Noradrenaline/Norepinephrine) ELISA Kit
中文名称：去甲肾上腺素(NA/NE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用NA/NE抗原包被于酶标板上，实验时样品或标准品中的NA/NE与包被的NA/NE竞争生物素标记的抗NA/NE单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，NA/NE浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中NA/NE的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的NA/NE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Chronic Blockade of NMDAR Subunit 2A in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Suppression of MEK/ERK/CREB Pathway
IF: 2.689 -
Effects of norepinephrine on colonic tight junction protein expression during heat stress
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：33747161Reactivity：RatSample Type：Plasma -
Norepinephrine-stimulated HSCs secrete sFRP1 to promote HCC progression following chronic stress via augmentation of a Wnt16B/β-catenin positive feedback loop
IF: 7.068Journal： JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2020)PMID：32293507Reactivity：HumanSample Type：Serum,Cell culture supernatant -
Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors
IF: 6.018Journal： Computational and Structural Biotechnology Journal (2020)PMID：33304464Reactivity：HumanSample Type：blood -
Comparison of high-fat style diet-induced dysregulation of baroreflex control of renal sympathetic nerve activity in intact and ovariectomized female rats
IF: 3.139 -
Lilium davidii extract alleviates p?chlorophenylalanine?induced insomnia in rats through modification of the hypothalamic-related neurotransmitters, melatonin and homeostasis of the hypothalamic-pituitary-adrenal axis
IF: 2.971 -
Activation of Paraventricular Melatonin Receptor 2 Mediates Melatonin-Conferred Cardioprotection Against Myocardial Ischemia/Reperfusion Injury
IF: 2.598Journal： JOURNAL OF CARDIOVASCULAR PHARMACOLOGY (2020)PMID：32433359Reactivity：MouseSample Type：plasma -
Anxiolytic and antioxidant effects of Euterpe oleracea Mart. (a?aí) seed extract in adult rat offspring submitted to periodic maternal separation
IF: 2.522Journal： Applied Physiology Nutrition and Metabolism (2020)Reactivity：RatSample Type：adrenal tissue -
Effects of botulinum toxin A on endometriosis?associated pain and its related mechanism Retraction in /10.3892/mmr.2020.11674
IF: 2.100Journal： Molecular Medicine Reports (2020)PMID：33000241Reactivity：MouseSample Type：Cell culture supernatant,Tissue homogenate -
Oxidative stress and neuromodulatory effects of deltamethrin and its combination with insect repellents in rats
IF: 2.649
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