NA/NE(Noradrenaline/Norepinephrine) ELISA Kit
中文名称:去甲肾上腺素(NA/NE)酶联免疫吸附测定试剂盒

Price:
- 反应性: Universal
- 检测范围: 0.31-20 ng/mL
- 灵敏度: 0.19 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用NA/NE抗原包被于酶标板上,实验时样品或标准品中的NA/NE与包被的NA/NE竞争生物素标记的抗NA/NE单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,NA/NE浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中NA/NE的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 2 h 30 min |
反应性 | Universal |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 0.31-20 ng/mL |
灵敏度 | 0.19 ng/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的NA/NE,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Differentiation of human glioblastoma U87 cells into cholinergic neuron
IF: 2.173Journal: NEUROSCIENCE LETTERS (2019)PMID:30928478Reactivity:HumanSample Type:Cell culture supernatant -
Effects of leptin on norepinephrine in acute ischemic stroke
IF: 0.820 -
Renal Denervation Effects on Myocardial Fibrosis and Ventricular Arrhythmias in Rats with Ischemic Cardiomyopathy
IF: 5.500Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2018)PMID:29742489Reactivity:RatSample Type:Plasma -
Norepinephrine stimulation downregulates the β2-adrenergic receptor–nitric oxide pathway in human pulmonary artery endothelial cells
IF: 3.923 -
High frequency deep transcranial magnetic stimulation acutely increases β-endorphins in obese humans
IF: 3.179 -
Selective ablation of the ligament of Marshall attenuates atrial electrical remodeling in a short-term rapid atrial pacing canine model
IF: 2.873Journal: JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY (2018)PMID:29864240Reactivity:CanineSample Type:Serum -
Selective ablation of the ligament of Marshall reduces ischemia and reperfusion-induced ventricular arrhythmias
IF: 2.766 -
Atrial Fibrillation in Acute Obstructive Sleep Apnea: Autonomic Nervous Mechanism and Modulation
IF: 4.425 -
Adjunctive therapy with statins reduces residual albuminuria/proteinuria and provides further renoprotection by downregulating the angiotensin II–AT1 pathway in hypertensive nephropathy
IF: 4.085 -
The Locus Coeruleus–Norepinephrine System Mediates Empathy for Pain through Selective Up-Regulation of P2X3 Receptor in Dorsal Root Ganglia in Rats
IF: 3.005

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