NA/NE(Noradrenaline/Norepinephrine) ELISA Kit
中文名称：去甲肾上腺素(NA/NE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用NA/NE抗原包被于酶标板上，实验时样品或标准品中的NA/NE与包被的NA/NE竞争生物素标记的抗NA/NE单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，NA/NE浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中NA/NE的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的NA/NE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Effects of constant darkness on behaviour and physiology of male and female mice
IF: 3.400 -
Constitutive Serotonin Tone as a Modulator of Brown Adipose Tissue Thermogenesis: A Rat Study
IF: 3.2 -
Hypoxia promotes white adipose tissues browning in rats under simulated environment at altitude of 5000?m
IF: 3.100Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023)PMID：37187092Reactivity：RatSample Type：plasma -
Anti-Inflammatory Effects of Two-Week Sacral Nerve Stimulation Therapy in Patients With Ulcerative Colitis
IF: 2.800 -
Chronic unpredictable mild stress promotes atherosclerosis via adipose tissue dysfunction in ApoE-/- mice
IF: 2.7 -
Propofol combined with alfentanil for general anesthesia in vocal cord polypectomy under suspension laryngoscopy
IF: 2.2Journal： American Journal of Translational Research (2023)DOI：PMID:38186970PMID：38186970Reactivity：HumanSample Type：plasma -
The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial
IF: 1.600Journal： Journal of General and Family Medicine (2023)DOI：10.1002/jgf2.606PMID：37261039Reactivity：HumanSample Type：serum -
Imipramine activates FAM3A-FOXA2-CPT2 pathway to ameliorate hepatic steatosis
IF: 13.934Journal： METABOLISM-CLINICAL AND EXPERIMENTAL (2022)PMID：35995281Reactivity：MouseSample Type：serum -
Spinal cord astrocytes regulate myocardial ischemia–reperfusion injury
IF: 12.416 -
Neuroendocrine stress hormones associated with short-term exposure to nitrogen dioxide and fine particulate matter in individuals with and without chronic obstructive pulmonary disease: A panel study in Beijing, China
IF: 9.988
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