NA/NE(Noradrenaline/Norepinephrine) ELISA Kit
中文名称：去甲肾上腺素(NA/NE)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.19 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用NA/NE抗原包被于酶标板上，实验时样品或标准品中的NA/NE与包被的NA/NE竞争生物素标记的抗NA/NE单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，NA/NE浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中NA/NE的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.19 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的NA/NE，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Activation of locus coeruleus-spinal cord?noradrenergic neurons alleviates neuropathic pain in mice via reducing neuroinflammation from astrocytes and microglia in spinal dorsal horn
IF: 9.587Journal： Journal of Neuroinflammation (2022)PMID：35624514Reactivity：MouseSample Type：spinal cord tissue -
Protective Effect of a Cocoa-Enriched Diet on Oxidative Stress Induced by Intensive Acute Exercise in Rats
IF: 7.675 -
Uncoupling Protein-1 Modulates Anxiety-Like Behavior in a Temperature-Dependent Manner
IF: 6.709 -
Maternal anxiety affects embryo implantation via impairing adrenergic receptor signaling in decidual cells
IF: 6.548 -
Influence of Diets Enriched with Flavonoids (Cocoa and Hesperidin) on the Systemic Immunity of Intensively Trained and Exhausted Rats
IF: 6.064 -
Integrated Functional Neuroimaging, Monoamine Neurotransmitters, and Behavioral Score on Depressive Tendency in Intensive Care Unit Medical Staffs Induced by Sleep Deprivation After Night Shift Work
IF: 5.435 -
Efficacy of a novel proprietary dietary supplement (TRI 360TM) on psychological symptoms and stress-related quality of life in adult subjects: A randomized controlled clinical trial
IF: 5.435 -
New insights into the central sympathetic hyperactivity post-myocardial infarction: Roles of METTL3-mediated m6A methylation
IF: 5.295Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022)PMID：35040253Reactivity：RatSample Type：serum,myocardium tissue -
Effect of TLR4/MyD88/NF-kB axis in paraventricular nucleus on ventricular arrhythmias induced by sympathetic hyperexcitation in post-myocardial infarction rats
IF: 5.295Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022)PMID：35393774Reactivity：RatSample Type：Plasma , myocardial tissue -
The Role of the Superior Cervical Sympathetic Ganglion in Ischemia Reperfusion-Induced Acute Kidney Injury in Rats
IF: 5.058
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