OT(Oxytocin) ELISA Kit
中文名称:催产素(OT)酶联免疫吸附测定试剂盒

Price:
- 反应性: Universal
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用OT抗原包被于酶标板上,实验时样品或标准品中的OT与包被的OT竞争生物素标记的抗OT单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,OT浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中OT的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 2 h 30 min |
反应性 | Universal |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的OT,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Effects of combined postweaning social isolation and ketamine administration on schizophrenia-like behaviour in male Sprague Dawley rats
IF: 2.600Journal: BEHAVIOURAL BRAIN RESEARCH (2024)Reactivity:RatSample Type:Amygdala tissue -
Withania somnifera ameliorates sexual arousal and impotence in stressed sexually sluggish male rats by modulating neurotransmitters and NO/cGMP/PDE5α pathway
IF: 5.400 -
The Effects of Chronic Asprosin Administration on Sense of Smell and Sexual Behavior in Female Rats
IF: 4.100 -
Prolonged Ketamine Therapy Differentially Rescues Psychobehavioural Deficits Via Modulation of Nitro-oxidative Stress and Oxytocin Receptors in the Gut-brain-axis of Depressed Mice
IF: 3.700 -
Large litter size increases oxidative stress and adversely affects nest-building behavior and litter characteristics in primiparous sows
IF: 3.200 -
Skin-to-skin contact-An effective intervention on pain and stress reduction in preterm infants
IF: 2.600 -
The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial
IF: 1.600Journal: Journal of General and Family Medicine (2023)DOI:10.1002/jgf2.606PMID:37261039Reactivity:HumanSample Type:serum -
Abortion associated with postpartum opportunistic bacterial invasion reduces fertility and induces disturbances of reproductive hormones, hematological profile, and oxidant/antioxidant profiles in dairy cows
IF: 1.400Journal: Journal of Advanced Veterinary and Animal Research (2023)Reactivity:BovineSample Type:Blood sample -
Oxytocin Nanogels Inhibit Innate Inflammatory Response for Early Intervention in Alzheimer’s Disease
IF: 10.383 -
Protective Effects of Intranasally Administrated Oxytocin-Loaded Nanoparticles on Pentylenetetrazole-Kindling Epilepsy in Terms of Seizure Severity, Memory, Neurogenesis, and Neuronal Damage
IF: 5.780