OT(Oxytocin) ELISA Kit
中文名称：催产素(OT)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用OT抗原包被于酶标板上，实验时样品或标准品中的OT与包被的OT竞争生物素标记的抗OT单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，OT浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中OT的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的OT，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance
IF: 1.660 -
Variability of serum reproductive hormones in cows presenting various reproductive conditions in semi-arid areas of the North West Province, South Africa
-
Social isolation rearing-induced anxiety and response to agomelatine in male and female rats: Role of corticosterone, oxytocin, and vasopressin
IF: 4.221 -
Protective effect of oxytocin on LPS-induced acute lung injury in mice
IF: 4.011 -
Regular Aerobic Voluntary Exercise Increased Oxytocin in Female Mice: The Cause of Decreased Anxiety and Increased Empathy-Like Behaviors
IF: 1.203 -
The effects of acute foot shock stress on empathy levels in rats
IF: 3.173Journal： BEHAVIOURAL BRAIN RESEARCH (2018)PMID：29709611Reactivity：RatSample Type：Serum,Tissue homogenate -
Acetaminophen (paracetamol) affects empathy-like behavior in rats: Dose-response relationship
IF: 2.538Journal： PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR (2018)PMID：30359628Reactivity：RatSample Type：Serum,Tissue homogenate -
Evaluation of behavior and endocrinological changes after REM sleep deprivation-induced mania-like behavior in mice
IF: 0.655Journal： Sleep and Biological Rhythms (2018)Reactivity：MouseSample Type：serum -
Immediate breastfeeding and skin-to-skin contact during cesarean section decreases maternal oxidative stress, a prospective randomized case-controlled study
IF: 1.367
实验操作视频
