PE Anti-Mouse IL-17A Antibody[TC11-18H10.1]（E-AB-F1199D）|-Elabscience伊莱瑞特【官网】

PE Anti-Mouse IL-17A Antibody[TC11-18H10.1]

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货号：E-AB-F1199D
文献（11）
说明书

说明书

PE Anti-Mouse IL-17A Antibody[TC11-18H10.1]

Price: ¥1080.00 ¥648.00 ¥1656.00

Size:

100 Tests 50 Tests 100 Tests × 2

应用性： ICFCM
亚型： Rat IgG1, κ
宿主： Rat

流式标记抗体&同型对照抗体特惠65折（不含流式纯抗）

2025-08-19 ~ 2025-09-30

Alternate Names	CTLA-8, CTLA8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, Interleukin-17A
Cellular Localization	Secreted
Clone No	TC11-18H10.1
Isotype	Rat IgG1, κ
Leadtime	咨询
Background	IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.
Abbre	IL-17A
Swissprot	Q62386
Host	Rat
Reactivity	Mouse
Clonality	Monoclonal
Isotype Control	PE Rat IgG1, κ Isotype Control[HRPN]
Applications	ICFCM
Form	Liquid
Concentration	5 μL/Test
Conjugation	PE
Conjugation Information	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
Spectrum
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
Storage	This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Expiration Date	12个月
Shipping	冰袋
Gene ID	16171

HEK293T cells transiently transfected with pcDNA3.1 plasmid encoding Mouse IL-17A gene are stained with PE Anti-Mouse IL-17A Antibody (filled gray histogram) or PE Rat IgG1, κ lsotype Control (empty black histogram).

Dendrobium fimbriatum Hook polysaccharide ameliorates dextran-sodium-sulfate-induced colitis in mice via improving intestinal barrier function, modulating intestinal microbiota, reducing oxidative stress and inflammatory responses

IF: 5.396

Journal： Food & Function (2021)

DOI：10.1039/D1FO03003E

PMID：34874039

Reactivity：Mouse

E-CK-A105	10×ACK Lysis Buffer	500 mL , 200 mL , 100 mL
E-CK-A107	Cell Staining Buffer	500 mL , 200 mL , 100 mL
E-CK-A109	Intracellular Fixation/Permeabilization Buffer Kit	500 Assays , 100 Assays , 50 Assays
E-CK-A091	Cell Stimulation and Protein Transport Inhibitor Kit	500 Assays , 200 Assays , 100 Assays , 50 Assays

Q11:IHC实验，想知道抗原修复条件是什么？是用EDTA还是枸橼酸钾？

修复条件一般为高温高压或者微波加热修复。
例如：抗原修复（高压法）：压力锅中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液（pH 6.0）（配制：将抗原修复液溶于相应体积 ddH2O中，混匀），加热至沸腾，切片置耐热塑料切片架上，放入锅中，盖好锅盖，扣上压力阀，继续加热，设置保压 4 min，时间到后打开放气阀放气，压力归零后开锅盖将内锅取出放置室温冷却，待溶液冷却至室温后取出切片（约30 min）。
抗原修复（微波法）：烧杯中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液（pH 6.0）（配制：将抗原修复液溶于相应体积 ddH2O中，混匀），加热至沸腾，切片置耐热塑料切片架上，放入烧杯中，中小火加热30min后取出，放置室温冷却，待溶液冷却至室温后取出切片（约30 min）。
IHC修复液的选择建议参考抗体使用说明书，或者参考以下方案：人体样本，优先推荐使用pH9.0的EDTA修复液（临床样本）；大/小鼠样本，优先推荐使用pH6.0的枸橼酸修复液（科研样本）。

Q12:IHC在数据表上是什么意思？是冷冻切片吗？与IHC-F区别？

IHC是免疫组化实验，使用的切片一般包含有IHC-P（石蜡切片）和IHC-F（冰冻切片）两种。