PGE2(Prostaglandin E2) ELISA Kit
中文名称：前列腺素E2(PGE2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用PGE2抗原包被于酶标板上，实验时样品或标准品中的PGE2与包被的PGE2竞争生物素标记的抗PGE2单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，PGE2浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中PGE2的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的PGE2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
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Phillygenin inhibited LPS-induced RAW 264.7?cell inflammation by NF-κB pathway
IF: 4.432Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2021)PMID：33745957Reactivity：MouseSample Type：Cell culture supernatant -
The protective effects of allopurinol against IL-17A-induced inflammatory response in mast cells
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Vitamin D stimulates miR-26b-5p to inhibit placental COX-2 expression in preeclampsia
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Protective effect of the combination of essential oil from patchouli and tangerine peel against gastric ulcer in rats
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Benzoylaconine Modulates LPS-Induced Responses Through Inhibition of Toll-Like Receptor-Mediated NF-κB and MAPK Signaling in RAW264.7 Cells
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Dexamethasone-loaded thermo-sensitive hydrogel attenuates osteoarthritis by protecting cartilage and providing effective pain relief
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Effect of Telmisartan on Arsenic-Induced (Sub-chronic) Perturbations in Redox Homeostasis, Pro-inflammatory Cascade and Aortic Dysfunction in Wistar Rats
IF: 3.738 -
Thymol ameliorates 5-fluorouracil-induced intestinal mucositis: Evidence of down-regulatory effect on TGF-β/MAPK pathways through NF-κB
IF: 3.652Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2021)PMID：34665902Reactivity：RatSample Type：serum, intestianl tissue -
(3α)-3-(tiglinoyloxy)-ent-kaur-16-en-19-oic acid, isolated from Wedelia trilobata L., exerts an anti-inflammatory effect via the modulation of NF-κB, MAPK and mTOR pathway and autophagy in LPS-stimulated macrophages
IF: 3.500Journal： TOXICOLOGY IN VITRO (2021)PMID：33705895Reactivity：mouseSample Type：Cell culture supernatant -
A biolabel research based on metabonomics reveals the therapeutic potentials of Herba Lysimachiae in synovial diseases: The dual effects on synovial platelet aggregation by prostaglandin E1/E2
IF: 3.205Journal： JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2021)PMID：33957357Reactivity：RatSample Type：Tissue homogenate(synovial)
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