PGE2(Prostaglandin E2) ELISA Kit
中文名称：前列腺素E2(PGE2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Universal
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用PGE2抗原包被于酶标板上，实验时样品或标准品中的PGE2与包被的PGE2竞争生物素标记的抗PGE2单抗上的结合位点，游离的成分被洗去。加入辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，PGE2浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中PGE2的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 2 h 30 min |
| 反应性 | Universal |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的PGE2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Limonin modulated immune and inflammatory responses to suppress colorectal adenocarcinoma in mice model
IF: 3.000Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2021)PMID：34009457Reactivity：MouseSample Type：Serum -
α?rhamnrtin?3?α?rhamnoside exerts anti?inflammatory effects on lipopolysaccharide?stimulated RAW264.7 cells by abrogating NF?κB and activating the Nrf2 signaling pathway
IF: 2.952 -
Gastroprotective effect of hydromethanolic extract of Ayapana triplinervis leaves on indomethacin-induced gastric ulcer in male Wistar rats
IF: 2.720 -
Gallic acid and myricetin-rich Labisia pumila extract mitigated multiple diabetic eye disorders in rats
IF: 2.720Journal： JOURNAL OF FOOD BIOCHEMISTRY (2021)Reactivity：RatSample Type：serum -
Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7)
IF: 2.699 -
Synthesis and anti-inflammatory activity of novel steroidal chalcones with 3β-pregnenolone ester derivatives in RAW 264.7 cells in vitro
IF: 2.668 -
Proteomic Analysis and In Vivo Studies Reveal the Potential Gastroprotective Effects of CHCl3 and Aqueous Extracts of Ficus palmata
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：34194521Reactivity：RatSample Type：Tissue homogenate -
The Anti-Inflammatory Effect of Smilax china L. Extract on LPS-Stimulated THP-1 via Downregulation of MAPK and NF- κ B Signaling Pathway
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：34824594Reactivity：Human -
Study on the inhibition of inflammation by the cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2) pathway and the promotion of wound healing of oral ulcer of Yangyin Shengji powder after chemotherapy
IF: 2.595Journal： Annals of Palliative Medicine (2021)Reactivity：MouseSample Type：mucosal tissue -
Vicenin?3 ameliorates ECM degradation by regulating the MAPK pathway in SW1353 chondrocytes
IF: 2.447
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
