Porcine IgG(Immunoglobulin G) ELISA Kit
中文名称：猪免疫球蛋白G(IgG)酶联免疫吸附测定试剂盒
Price:
- 反应性： Porcine
- 检测范围： 3.13-200 ng/mL
- 灵敏度： 1.88 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗猪IgG抗体包被于酶标板上，实验时样品（或标准品）中的猪IgG会与包被抗体结合。后依次加入生物素化的抗猪IgG抗体和辣根过氧化物酶标记的亲和素，抗猪IgG抗体与结合在包被抗体上的猪IgG结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IgG浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IgG的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 4 h 30 min |
| 反应性 | Porcine |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 3.13-200 ng/mL |
| 灵敏度 | 1.88 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的猪IgG，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Detection and Analysis of Antidiarrheal Genes and Immune Factors in Various Shanghai Pig Breeds
IF: 4.800 -
Effect of sex and birth weight on the performance, microbiome, immune response and colostrum intake of piglets
IF: 1.800 -
The Effect of Supplementation with β-Hydroxy-β-Methylbutyric Acid (HMB) to Pregnant Sows on the Mucosal Structure, Immunolocalization of Intestinal Barrier Proteins, VIP and Leptin in the Large Intestine in their Offspring
IF: 1.900Journal： Annals of Animal Science (2023)Reactivity：PigSample Type：serum -
Weaning causes a prolonged but transient change in immune gene expression in the intestine of piglets
IF: 3.159 -
Fermented rye with Agaricus subrufescens and mannan-rich hydrolysate based feed additive to modulate post-weaning piglet immune response
IF: 3.048 -
Effects of starch and gelatin encapsulated vitamin A on growth performance, immune status and antioxidant capacity in weaned piglets
IF: 4.492 -
Effects of Dietary Supplementation of Humic Acid Sodium and Zinc Oxide on Growth Performance, Immune Status and Antioxidant Capacity of Weaned Piglets
IF: 2.323 -
Curcumin and Resveratrol Regulate Intestinal Bacteria and Alleviate Intestinal Inflammation in Weaned Piglets
IF: 3.060 -
Dietary Supplementation of Astragalus Polysaccharides Enhanced Immune Components and Growth Factors EGF and IGF-1 in Sow Colostrum
IF: 3.276 -
Effects of composite antimicrobial peptide on growth performance and health in weaned piglets
IF: 1.325
实验操作视频
