Porcine TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称：猪肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Porcine
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗猪TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的猪TNF-α会与包被抗体结合。后依次加入生物素化的抗猪TNF-α抗体和辣根过氧化物酶标记的亲和素，抗猪TNF-α抗体与结合在包被抗体上的猪TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Porcine |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的猪TNF-α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Detection and Analysis of Antidiarrheal Genes and Immune Factors in Various Shanghai Pig Breeds
IF: 4.800 -
Effect of sex and birth weight on the performance, microbiome, immune response and colostrum intake of piglets
IF: 1.800 -
Abnormal Fetal Lung of Hoxa1?/? Piglets Is Rescued by Maternal Feeding with All-Trans Retinoic Acid
IF: 3.000 -
Clinical improvement of sepsis by extracorporeal centrifugal leukocyte apheresis in a porcine model
IF: 8.440 -
Dietary dimethylglycine sodium salt supplementation alleviates redox status imbalance and intestinal dysfunction in weaned piglets with intrauterine growth restriction
IF: 5.285 -
Effects of oral of administration of monoglycide laurate on virus load and inflammation in PEDV infected porcine
IF: 3.471Journal： Frontiers in Veterinary Science (2022)Reactivity：PigSample Type：serum -
Dietary ferulic acid and vanillic acid on inflammation, gut barrier function and growth performance in lipopolysaccharide-challenged piglets
IF: 6.383 -
Preclinical efficacy study of a porous biopolymeric scaffold based on gelatin-hyaluronic acid-chondroitin sulfate in a porcine burn injury model: role of critical molecular markers (VEGFA, N-cadherin, COX-2), gamma sterilization efficacy and a comparison of healing potential to Integra?
IF: 3.715 -
Dietary protocatechuic acid ameliorates inflammation and up-regulates intestinal tight junction proteins by modulating gut microbiota in LPS-challenged piglets
IF: 4.167Journal： Journal of Animal Science and Biotechnology (2020)PMID：32944233Reactivity：PigSample Type：Serum -
Koumine Alleviates Lipopolysaccharide-Induced Intestinal Barrier Dysfunction in IPEC-J2 Cells by Regulating Nrf2/NF-κB Pathway
IF: 3.682Journal： AMERICAN JOURNAL OF CHINESE MEDICINE (2020)PMID：31931594Reactivity：PigSample Type：Cell culture medium
实验操作视频
