Purified Anti-Mouse CD16/32 Antibody[2.4G2]（E-AB-F0997A）|-Elabscience伊莱瑞特【官网】

Purified Anti-Mouse CD16/32 Antibody[2.4G2]

一键复制产品信息

货号：E-AB-F0997A
文献（56）
说明书

说明书

Purified Anti-Mouse CD16/32 Antibody[2.4G2]

Price: ¥400.00 ¥180.00

Size:

100 μg 25 μg

应用性： FCM;Block
亚型： Rat IgG2b, κ
宿主： Rat

Alternate Names	CD16a/b, CD32, CD32A/B, FCG2A, FCGR2A/BFCGR3, FCGR3A/B, Fc fragment of IgG low affinity IIIa/b receptor, Fc fragment of IgG low affinity IIIb receptor, Fc fragment of IgG low affinity IIa/b receptor, Fc gamma RIIa/b , Fc gamma receptor III A/B, FcGR
Clone No	2.4G2
Isotype	Rat IgG2b, κ
Leadtime	咨询
Verified Samples	Verified Samples in FCM: C57/BL6 Mouse splenocytes
Dilution	FCM 2 μg/mL(1×10⁵-5×10⁵ cells)
Background	CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.
Immunogen	Recombinant Mouse CD16/32 protein
Abbre	CD16/32
Swissprot	P08508, P08101
Host	Rat
Reactivity	Mouse
Clonality	Monoclonal
Purification Method	>98%, Protein A/G purified
Applications	FCM;Block
Research Areas	Immunology;Innate Immunity
Concentration	≥ 1 mg/mL
Conjugation	Unconjugated
Storage Buffer	Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Storage	Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
Expiration Date	12个月
Shipping	Ice bag

C57/BL6 Mouse splenocytes were stained with 0.2 μg Purified Anti-Mouse CD16/32 Antibody[2.4G2](Right) and 0.2 μg Rat IgG2b, κ Isotype Control(Left), followed by Alexa Fluor^® 647-conjugated Goat Anti- Rat IgG Secondary Antibody, then anti-Mouse B220 PE-conjugated Monoclonal Antibody.

Myeloid deficiency of Z‐DNA binding protein 1 restricts septic cardiomyopathy via promoting macrophage polarisation towards the M2‐subtype

IF: 7.9

Journal： Clinical and Translational Medicine (2025)

DOI：10.1002/ctm2.70315

Reactivity：Mouse
Identification of a Novel Cuproptosis Inducer That Induces ER Stress and Oxidative Stress to Trigger Immunogenic Cell Death in Tumors

IF: 7.1

Journal： FREE RADICAL BIOLOGY AND MEDICINE (2025)

DOI：10.1016/j.freeradbiomed.2025.01.042

Reactivity：Mouse
A KIF20A-based thermosensitive hydrogel vaccine effectively potentiates immune checkpoint blockade therapy for hepatocellular carcinoma

IF: 6.9

Journal： npj Vaccines (2025)

DOI：10.1038/s41541-024-01060-2

Reactivity：Mouse
Puerarin mitigates cognitive decline and white matter injury via CD36-Mediated microglial phagocytosis in chronic cerebral hypoperfusion

IF: 6.7

Journal： PHYTOMEDICINE (2025)

DOI：10.1016/j.phymed.2025.156396

Reactivity：Mouse
Activated Notch1 promotes macrophage polarization and exacerbates sepsis-induced acute lung injury via β-catenin/NF-κB signaling

IF: 5.3

Journal： BIOCHEMICAL PHARMACOLOGY (2025)

DOI：10.1016/j.bcp.2025.116892

Reactivity：Mouse
Dietary Fiber Intake Improves Osteoporosis Caused by Chronic Lead Exposure by Restoring the Gut–Bone Axis

IF: 4.8

Journal： Nutrients (2025)

DOI：10.3390/nu17091513

Reactivity：Mouse
Regulation of Host Regulatory T Cell Differentiation by emu-let-7-5p in Echinococcus multilocularis Infection Through Targeting NFκB2

IF: 4.4

Journal： FASEB JOURNAL (2025)

DOI：10.1096/fj.202403437R

Reactivity：Mouse
The browning and mobilization of subcutaneous white adipose tissue supports efficient skin repair

IF: 27.7

Journal： Cell Metabolism (2024)

DOI：10.1016/j.cmet.2024.05.005

Reactivity：Mouse
Nanomedicines Promote Cartilage Regeneration in Osteoarthritis by Synergistically Enhancing Chondrogenesis of Mesenchymal Stem Cells and Regulating Inflammatory Environment

IF: 15.8

Journal： ACS Nano (2024)

DOI：10.1021/acsnano.3c11848

Reactivity：Mouse
CD276-dependent efferocytosis by tumor-associated macrophages promotes immune evasion in bladder cancer

IF: 14.7

Journal： Nature Communications (2024)

DOI：10.1038/s41467-024-46735-5

Reactivity：Mouse

Sample Type：bladder tissue

E-AB-F0997M	Elab Fluor® 647 Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997L	Elab Fluor® 488 Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997I	PE/Cyanine5.5 Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997J	PerCP/Cyanine5.5 Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997G	PE/Cyanine5 Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997H	PE/Cyanine7 Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997E	APC Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997C	FITC Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997D	PE Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997B	Biotin Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F09970	AF/LE Purified Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997UE	APC Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997UD	PE Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997UC	FITC Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997UJ	PerCP/Cyanine5.5 Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997UI	PE/Cyanine5.5 Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997UH	PE/Cyanine7 Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997UG	PE/Cyanine5 Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997UM	Elab Fluor® 647 Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997UL	Elab Fluor® 488 Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997S	Elab Fluor® Red 780 Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997Q	Elab Fluor® Violet 450 Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997UQ	Elab Fluor® Violet 450 Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997US	Elab Fluor® Red 780 Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg
E-AB-F0997P	PE/Elab Fluor® 594 Anti-Mouse CD16/32 Antibody[2.4G2]	100 Tests , 50 Tests , 100 Tests × 2
E-AB-F0997UP	PE/Elab Fluor® 594 Anti-Mouse CD16/32 Antibody[2.4G2]	100 μg , 25 μg

Q11:IHC实验，想知道抗原修复条件是什么？是用EDTA还是枸橼酸钾？

修复条件一般为高温高压或者微波加热修复。
例如：抗原修复（高压法）：压力锅中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液（pH 6.0）（配制：将抗原修复液溶于相应体积 ddH2O中，混匀），加热至沸腾，切片置耐热塑料切片架上，放入锅中，盖好锅盖，扣上压力阀，继续加热，设置保压 4 min，时间到后打开放气阀放气，压力归零后开锅盖将内锅取出放置室温冷却，待溶液冷却至室温后取出切片（约30 min）。
抗原修复（微波法）：烧杯中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液（pH 6.0）（配制：将抗原修复液溶于相应体积 ddH2O中，混匀），加热至沸腾，切片置耐热塑料切片架上，放入烧杯中，中小火加热30min后取出，放置室温冷却，待溶液冷却至室温后取出切片（约30 min）。
IHC修复液的选择建议参考抗体使用说明书，或者参考以下方案：人体样本，优先推荐使用pH9.0的EDTA修复液（临床样本）；大/小鼠样本，优先推荐使用pH6.0的枸橼酸修复液（科研样本）。

Q12:IHC在数据表上是什么意思？是冷冻切片吗？与IHC-F区别？

IHC是免疫组化实验，使用的切片一般包含有IHC-P（石蜡切片）和IHC-F（冰冻切片）两种。