QuicKey Pro Human Cortisol ELISA Kit
中文名称:QuicKey Pro-人皮质醇(Cortisol)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 6.25-400 ng/mL
- 灵敏度: 2.87 ng/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测人血清、血浆、唾液、尿液中Cortisol浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用人Cortisol抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗Cortisol单克隆抗体,样本(或标准品中)的Cortisol与包被抗原Cortisol竞争结合HRP标记的抗Cortisol单抗上的结合位点,形成包被抗原Cortisol-HRP标记的抗Cortisol单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Cortisol浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中Cortisol的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 1 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 6.25-400 ng/mL |
灵敏度 | 2.87 ng/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆、唾液、尿液 |
特异性 | 可检测样本中的人Cortisol,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 6个月 |
数据处理 |
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Dietary Supplementation with an Extract of Aloysia citrodora (Lemon verbena) Improves Sleep Quality in Healthy Subjects: A Randomized Double-Blind Controlled Study
IF: 4.800 -
CCR3 knockdown attenuates prolonged underwater operations-induced cognitive impairment via alleviating microglia-mediated neuroinflammation
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A randomized, double-blinded, placebo-controlled clinical trial on Lactobacillus-containing cultured milk drink as adjuvant therapy for depression in irritable bowel syndrome
IF: 3.800Journal: Scientific Reports (2024)Reactivity:HumanSample Type:serum -
A feasibility study on indoor therapeutic horticulture to alleviate sleep and anxiety problems: The impact of plants and activity choice on its therapeutic effect
IF: 3.600Journal: COMPLEMENTARY THERAPIES IN MEDICINE (2024)PMID:38452859Reactivity:HumanSample Type:saliva sample -
The Welfare of Horses Competing in Three-Barrel Race Events Is Shown to Be Not Inhibited by Short Intervals between Starts
IF: 3.000 -
The Impact of Fear of Cancer Recurrence on the Quality of Life in Breast Cancer Patients: A Longitudinal Study of the Mediation Effect of Cortisol and Hope
IF: 2.700Journal: European Journal of Oncology Nursing (2024)Reactivity:HumanSample Type:saliva -
Psychological and Biological Stress Pathways as Common Mechanisms Underlying a Psycho-Neurological Symptom Cluster in Cancer Patients: Perceived Stress, Cortisol, and ACTH
IF: 2.700Journal: European Journal of Oncology Nursing (2024)Reactivity:HumanSample Type:serum -
Hypothalamus-pituitary-adrenal axis in patients with post-traumatic stress disorders and related to oxidative stress
IF: 1.100Journal: Hormone Molecular Biology and Clinical Investigation (2024)Reactivity:HumanSample Type:serum -
The relationship between liver function and neurophysiological factors in depressed individuals: a cross-sectional study using an integrated “East meets West” medicine approach
IF: 4.700 -
Time Course of Performance Indexes, Oxidative Stress, Inflammation, and Muscle Damage Markers after a Female Futsal Match
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