QuicKey Pro Human Pg(Progesterone) ELISA Kit
中文名称:QuicKey Pro-人孕激素/孕酮(Pg)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 78.13-5000 pg/mL
- 灵敏度: 36.24 pg/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测人血清、血浆中Pg浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用人Pg抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗Pg单克隆抗体,样本(或标准品中)的Pg与包被抗原Pg竞争结合HRP标记的抗Pg单抗上的结合位点,形成包被抗原Pg-HRP标记的抗Pg单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Pg浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中Pg的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 1 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 78.13-5000 pg/mL |
灵敏度 | 36.24 pg/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的人Pg,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 6个月 |
数据处理 |
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Assessment Between Follicular Fluid Multiple Element Concentrations and Hormonal Profiles of Women with Polycystic Ovary Syndrome
IF: 6.700Journal: Exposure and Health (2023)Reactivity:HumanSample Type:blood -
Human umbilical cord mesenchymal stem cell-derived exosomes inhibit ovarian granulosa cells inflammatory response through inhibition of NF-κB signaling in polycystic ovary syndrome
IF: 3.993 -
Copper exposure disrupts ovarian steroidogenesis in human ovarian granulosa cells via the FSHR/CYP19A1 pathway and alters methylation patterns on the SF-1 gene promoter
IF: 4.374 -
Long-term in vitro exposure of human granulosa cells to the mixture of endocrine disrupting chemicals found in human follicular fluid disrupts steroidogenesis
IF: 3.500 -
Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy
IF: 14.976