QuicKey Pro Mouse CORT(Corticosterone) ELISA Kit
中文名称：QuicKey Pro-小鼠皮质酮(CORT)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 12.5-800 ng/mL
- 灵敏度： 4.98 ng/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆、唾液、尿液中Cort浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠Cort抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗Cort单克隆抗体，样本（或标准品中）的Cort与包被抗原Cort竞争结合HRP标记的抗Cort单抗上的结合位点，形成包被抗原Cort-HRP标记的抗Cort单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Cort浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中Cort的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 12.5-800 ng/mL |
| 灵敏度 | 4.98 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆、唾液、尿液 |
| 特异性 | 可检测样本中的小鼠Cort，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
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Psychological stress-induced microbial metabolite indole-3-acetate disrupts intestinal cell lineage commitment
IF: 29.000 -
The gut microbiome in patients with Cushing’s disease affects depression- and anxiety-like behavior in mice
IF: 13.800 -
The extracellular vesicle of depressive patient-derived Escherichia fergusonii induces vagus nerve-mediated neuroinflammation in mice
IF: 9.300Journal： Journal of Neuroinflammation (2024)Reactivity：MouseSample Type：colonic tissue,hippocampal tissue,sera -
Circadian light/dark cycle reversal exacerbates the progression of chronic kidney disease in mice
IF: 8.300 -
Glutamatergic neurons of basolateral amygdala mediate increased exploratory behaviors produced by mildly chronic restraint stress in adolescent mice
IF: 6.700Journal： PROGRESS IN NEUROBIOLOGY (2024)Reactivity：MouseSample Type：serum -
Contagion of depression: a double-edged sword
IF: 5.800Journal： Translational Psychiatry (2024)Reactivity：MouseSample Type：serum -
AdipoRon exerts an antidepressant effect by inhibiting NLRP3 inflammasome activation in microglia via promoting mitophagy
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
New polycyclic polyprenylated acylphloroglucinols with antidepressant activities from Hypericum perforatum L.
IF: 4.500Journal： BIOORGANIC CHEMISTRY (2024)Reactivity：MouseSample Type：serum -
Chronic sleep deprivation disturbs energy balance modulated by suprachiasmatic nucleus efferents in mice
IF: 4.400 -
Functional redundancy between glucocorticoid and mineralocorticoid receptors in mature corticotropin-releasing hormone neurons protects from obesity
IF: 4.200
