QuicKey Pro Mouse E2(Estradiol) ELISA Kit
中文名称：QuicKey Pro-小鼠雌二醇（Estradiol）酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.88 pg/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆、唾液、尿液中E2浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠E2抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗E2单克隆抗体，样本（或标准品中）的E2与包被抗原E2竞争结合HRP标记的抗E2单抗上的结合位点，形成包被抗原E2-HRP标记的抗E2单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，E2浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中E2的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.88 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆、唾液、尿液 |
| 特异性 | 可检测样本中的小鼠E2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
-
SHP2-Triggered Endothelial Cell Activation Fuels Estradiol-Independent Endometrial Sterile Inflammation
IF: 14.300 -
Inhibition of sympathetic tone via hypothalamic descending pathway propagates glucocorticoid-induced endothelial impairment and osteonecrosis of the femoral head
IF: 14.300Journal： Bone Research (2024)Reactivity：MouseSample Type：serum,femoral head bone marrow tissue -
Polystyrene nanoplastics induce apoptosis, autophagy, and steroidogenesis disruption in granulosa cells to reduce oocyte quality and fertility by inhibiting the PI3K/AKT pathway in female mice
IF: 10.600 -
Zinc finger protein ZNF638 regulates triglyceride metabolism via ANGPTL8 in an estrogen dependent manner
IF: 9.800Journal： METABOLISM-CLINICAL AND EXPERIMENTAL (2024)PMID：38211696Reactivity：MouseSample Type：serum -
Environmental copper exposure, placental cuproptosis, and miscarriage
IF: 8.900 -
Collagen (peptide) extracted from sturgeon swim bladder: Physicochemical characterization and protective effects on cyclophosphamide-induced premature ovarian failure in mice
IF: 8.500Journal： FOOD CHEMISTRY (2024)Reactivity：MouseSample Type：serum -
Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice
IF: 8.400Journal： Cell Communication and Signaling (2024)PMID：38491517Reactivity：MouseSample Type：plasma,prostate tissue -
YBX1 promotes type H vessel–dependent bone formation in an m5C-dependent manner
IF: 8.000 -
Silica nanoparticles cause ovarian dysfunction and fertility decrease in mice via oxidative stress-activated autophagy and apoptosis
IF: 6.200Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)Reactivity：MouseSample Type：Serum -
A heterozygous SPRY4 variant identified in female infertility characterized by reduced oocyte potential and early embryonic arrest
IF: 6.000
