QuicKey Pro Mouse E2(Estradiol) ELISA Kit
中文名称：QuicKey Pro-小鼠雌二醇（Estradiol）酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.88 pg/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆、唾液、尿液中E2浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠E2抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗E2单克隆抗体，样本（或标准品中）的E2与包被抗原E2竞争结合HRP标记的抗E2单抗上的结合位点，形成包被抗原E2-HRP标记的抗E2单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，E2浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中E2的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.88 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆、唾液、尿液 |
| 特异性 | 可检测样本中的小鼠E2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
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The effect of Bu Shen Huo Xue Tang on autoimmune premature ovarian insufficiency via Modulation of the Nrf2/Keap1 signaling pathway in mice
IF: 4.360 -
The Effect of Rooibos (Aspalathus linearis), Honeybush (Cyclopia intermedia) and Sutherlandia (Lessertia frutescens) on Testicular Insulin Signalling in Streptozotocin-Induced Diabetes in Wistar Rats
IF: 3.168Journal： Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2021)PMID：33776463Reactivity：RatSample Type：Plasma -
Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide.
IF: 2.916 -
Effect of Electroacupuncture on Reproductive Disorders and Insulin Resistance in a Murine Polycystic Ovary Syndrome Model
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：34987599Reactivity：MouseSample Type：serum -
Bisphenol A promotes autophagy in ovarian granulosa cells by inducing AMPK/mTOR/ULK1 signalling pathway
IF: 7.577Journal： ENVIRONMENT INTERNATIONAL (2020)PMID：33387880Reactivity：MouseSample Type：Cell culture supernatant, Serum -
Ovalbumin causes impairment of preimplantation embryonic growth in asthma-induced mice
IF: 4.018 -
Post-photostimulation energy intake accelerated pubertal development in broiler breeder pullets
IF: 2.659 -
Mechanisms by which electroacupuncture?mediated histone acetylation mitigates bone loss in rats with ovariectomy?induced osteoporosis
IF: 2.100 -
Hormone-balancing and protective effect of combined extract of?Sauropus androgynus and Elephantopus scaber against Escherichia?coli-induced renal and hepatic necrosis in pregnant mice
Journal： Journal of Ayurveda and Integrative Medicine (2020)PMID：32958334Reactivity：MouseSample Type：Serum -
Exposure to DEHP or its metabolite MEHP promotes progesterone secretion and inhibits proliferation in mouse placenta or JEG-3?cells
IF: 5.714Journal： ENVIRONMENTAL POLLUTION (2019)PMID：31771930Reactivity：MouseSample Type：Cell culture medium
