QuicKey Pro Mouse Pg(Progesterone) ELISA Kit
中文名称：QuicKey Pro-小鼠孕激素/孕酮(Pg)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 78.13-5000 pg/mL
- 灵敏度： 24.97 pg/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆中Pg浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠Pg抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗Pg单克隆抗体，样本（或标准品中）的Pg与包被抗原Pg竞争结合HRP标记的抗Pg单抗上的结合位点，形成包被抗原Pg-HRP标记的抗Pg单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Pg浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中Pg的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 78.13-5000 pg/mL |
| 灵敏度 | 24.97 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠Pg，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
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