QuicKey Pro Mouse T(Testosterone) ELISA Kit
中文名称：QuicKey Pro-小鼠睾酮(Testosterone)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.14 ng/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆中T浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠T抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗T单克隆抗体，样本（或标准品中）的T与包被抗原T竞争结合HRP标记的抗T单抗上的结合位点，形成包被抗原T-HRP标记的抗T单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，T浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中T的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.14 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠T，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
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Prenatal Silicon Dioxide Nanoparticles Exposure Reduces Female Offspring Fertility Without Affecting Males
IF: 14.300 -
Co-exposure of microcystin and nitrite enhanced spermatogenic disorders: The role of mtROS-mediated pyroptosis and apoptosis
IF: 10.300Journal： ENVIRONMENT INTERNATIONAL (2024)Reactivity：MouseSample Type：serum -
Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice
IF: 8.400Journal： Cell Communication and Signaling (2024)PMID：38491517Reactivity：MouseSample Type：plasma,prostate tissue -
Application of photocrosslinked gelatin, alginate and dextran hydrogels in the in vitro culture of testicular tissue
IF: 8.200Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)PMID：38232872Reactivity：MouseSample Type：Testes -
Chronic exposure to polystyrene microplastics induced LHR reduction and decreased testosterone levels through NF-κB pathway
IF: 7.600Journal： ENVIRONMENTAL POLLUTION (2024)Reactivity：MouseSample Type：serum -
Combined effects of polystyrene nanoplastics and lipopolysaccharide on testosterone biosynthesis and inflammation in mouse testis
IF: 6.800Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2024)PMID：38458071Reactivity：MouseSample Type：plasma,testes -
Retinoic acid mitigates the NSC319726-induced spermatogenesis dysfunction through cuproptosis-independent mechanisms
IF: 5.300Journal： CELL BIOLOGY AND TOXICOLOGY (2024)Reactivity：MouseSample Type：testes tissue -
Luteolin and its analog luteolin-7-methylether from Leonurus japonicus Houtt suppress aromatase-mediated estrogen biosynthesis to alleviate polycystic ovary syndrome by the inhibition of tumor progression locus 2
IF: 4.800Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum -
Chinese medicine compound prescription HeQi San ameliorates chronic inflammatory states and modulates gut flora in dehydroepiandrosterone-induced polycystic ovary syndrome mouse model
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：Serum -
A metabolomics approach reveals the pharmacological effects and mechanisms of Cistanche tubulosa stems and its combination with fluoxetine on depression in comorbid with sexual dysfunction
IF: 4.800Journal： JOURNAL OF ETHNOPHARMACOLOGY (2024)Reactivity：MouseSample Type：serum
Q1:我想测细胞培养上清里睾酮的含量，含酚红培养基会对睾酮检测有影响么?
含酚红的培养基可能对睾酮检测有影响。酚红在培养基中被用来作为PH值的指示剂：中性时为红色，酸性时为黄色，碱性时为紫色。研究表明，酚红可以模拟固醇类激素的作用（特别是雌激素）。为避免固醇类反应及对细胞的毒性作用，培养细胞，尤其是哺乳类细胞时，用不加酚红的培养基。
介绍一个小窍门：培养基在分装时，留一管加酚红，放到培养箱内平衡（可以看出PH值的变化，又可以看培养基是否污染）。在培养前放到培养箱内平衡再使用（可以避免培养时使用酚红）。
