QuicKey Pro Mouse T(Testosterone) ELISA Kit
中文名称：QuicKey Pro-小鼠睾酮(Testosterone)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.14 ng/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆中T浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠T抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗T单克隆抗体，样本（或标准品中）的T与包被抗原T竞争结合HRP标记的抗T单抗上的结合位点，形成包被抗原T-HRP标记的抗T单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，T浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中T的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.14 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠T，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
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Improvement of Late-Onset Hypogonadism Symptoms of Fermented Morinda citrifolia Extract in TM3 Leydig and TM4 Sertoli Cells
IF: 4.800 -
Effects of hyperhomocysteinemia on follicular development and oocytes quality
IF: 4.600 -
Cross-talk between oxidative stress and lipid metabolism regulators reveals molecular clusters and immunological characterization in polycystic ovarian syndrome
IF: 3.900Journal： Lipids in Health and Disease (2024)Reactivity：MouseSample Type：serum -
Knockdown of type 2 orexin receptor in adult mouse testis potentiates testosterone production and germ cell proliferation
IF: 3.800Journal： MOLECULAR AND CELLULAR ENDOCRINOLOGY (2024)Reactivity：MouseSample Type：serum,teste tissue -
Mixed exposure to PFOA and PFOS induces oocyte apoptosis and subfertility in mice by activating the Hippo signaling pathway
IF: 3.300Journal： REPRODUCTIVE TOXICOLOGY (2024)Reactivity：MouseSample Type：Serum -
Impact of chronic sleep deprivation on male reproductive health: Insights from testicular and epididymal responses in mice
IF: 3.200 -
Sub-acute bisphenol A exposure induces proteomic alterations and impairs male reproductive health in mice
IF: 3.200Journal： JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2024)Reactivity：MouseSample Type：serum -
Oligoasthenozoospermia is alleviated in a mouse model by [Gly14]-humanin-mediated attenuation of oxidative stress and ferroptosis
IF: 3.200 -
Comparing the pharmacological effects of the prepared folium of Epimedium brevicornu Maxim. and Epimedium sagittatum Maxim. on kidney-Yang deficiency syndrome and liver injury complications
IF: 2.500 -
Integrated stress response mediates HSP70 to inhibit testosterone synthesis in aging testicular Leydig cells
IF: 2.500Journal： Reproductive Biology (2024)Reactivity：MouseSample Type：testicular tissue
