QuicKey Pro Mouse T(Testosterone) ELISA Kit
中文名称：QuicKey Pro-小鼠睾酮(Testosterone)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.14 ng/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆中T浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠T抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗T单克隆抗体，样本（或标准品中）的T与包被抗原T竞争结合HRP标记的抗T单抗上的结合位点，形成包被抗原T-HRP标记的抗T单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，T浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中T的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.14 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠T，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
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Shenqi Qiangjing Granules Ameliorate Asthenozoospermia in Mice by Regulating Ferroptosis through the METTL3/GPX4 Signaling Axis
IF: 1.700Journal： TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE (2024)Reactivity：MouseSample Type：serum -
Dichloroacetic acid and trichloroacetic acid as disinfection by-products in drinking water are endocrine-disrupting chemicals
IF: 13.600 -
Sertoli cell-derived extracellular vesicles traverse the blood-testis barrier and deliver miR-24-3p inhibitor into germ cells improving sperm mobility
IF: 10.800 -
The Protective Effects Of Icariin Against Testicular Dysfunction In Type 1 Diabetic Mice Via AMPK-Mediated Nrf2 Activation And NF-κB p65 Inhibition
IF: 7.900 -
Based on network pharmacology to explore the effect and mechanism of Yipibushen decoction in improving obese type 2 diabetes mellitus with oligoasthenotspermia
IF: 5.400 -
Polystyrene nanoplastics inhibit StAR expression by activating HIF-1α via ERK1/2 MAPK and AKT pathways in TM3 Leydig cells and testicular tissues of mice
IF: 4.300Journal： FOOD AND CHEMICAL TOXICOLOGY (2023)PMID：36709824Reactivity：MouseSample Type：testis,serum -
Co-exposure to polystyrene microplastics and microcystin-LR aggravated male reproductive toxicity in mice
IF: 4.300 -
Fluorene-9-bisphenol exposure damages the testis in mice through a novel mechanism of ferroptosis
IF: 4.300 -
The long noncoding RNA CIRBIL is a regulator of steroidogenesis in mice
IF: 2.100 -
Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway
IF: 9.112
