QuicKey Pro Mouse T(Testosterone) ELISA Kit
中文名称：QuicKey Pro-小鼠睾酮(Testosterone)酶联免疫吸附测定试剂盒
Price:
- 反应性： Mouse
- 检测范围： 0.31-20 ng/mL
- 灵敏度： 0.14 ng/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测小鼠血清、血浆中T浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用小鼠T抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗T单克隆抗体，样本（或标准品中）的T与包被抗原T竞争结合HRP标记的抗T单抗上的结合位点，形成包被抗原T-HRP标记的抗T单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，T浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中T的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Mouse |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.31-20 ng/mL |
| 灵敏度 | 0.14 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的小鼠T，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
-
The protective effects of Procyanidin C-1 on bisphenol a-induced testicular dysfunction in aged mice
IF: 8.022Journal： Food Science and Human Wellness (2022)Reactivity：MouseSample Type：serum -
Effects of Cordyceps militaris fermentation products on reproductive development in juvenile male mice
IF: 4.996 -
Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice
IF: 4.982Journal： Reproductive Biology and Endocrinology (2022)PMID：35597951Reactivity：MouseSample Type：serum -
Fancd2os Reduces Testosterone Production by Inhibiting Steroidogenic Enzymes and Promoting Cellular Apoptosis in Murine Testicular Leydig Cells
IF: 3.607 -
Multiple transcriptomic profiling: potential novel metabolism-related genes predict prepubertal testis damage caused by DEHP exposure
IF: 4.223Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021)PMID：34595713Reactivity：MouseSample Type：serum -
DYRK1A Overexpression in Mice Downregulates the Gonadotropic Axis and Disturbs Early Stages of Spermatogenesis
IF: 4.096 -
Effect of Electroacupuncture on Reproductive Disorders and Insulin Resistance in a Murine Polycystic Ovary Syndrome Model
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：34987599Reactivity：MouseSample Type：serum -
Exposure to hyperandrogen drives ovarian dysfunction and fibrosis by activating the NLRP3 inflammasome in mice
IF: 6.551 -
Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis
IF: 5.778 -
Two resveratrol analogs, pinosylvin and 4,4′-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway
IF: 4.831
