QuicKey Pro Rat ADP/Acrp30(Adiponectin) ELISA Kit
中文名称:QuicKey Pro-大鼠脂联素(ADP/Acrp30)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 15.63-1000 ng/mL
- 灵敏度: 6.43 ng/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测大鼠血清、血浆中ADP/Acrp30浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠ADP/Acrp30抗体包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶标记的抗大鼠ADP/Acrp30检测抗体,样本(或标准品中)的大鼠ADP/Acrp30会与包被抗体及辣根过氧化物酶标记的抗大鼠ADP/Acrp30抗体结合,形成双抗体夹心的免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,ADP/Acrp30浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中ADP/Acrp30的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 1 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 ng/mL |
灵敏度 | 6.43 ng/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的大鼠ADP/Acrp30,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 6个月 |
数据处理 |
-
Anthocyanin-rich black wheat as a functional food for managing type 2 diabetes mellitus: a study on high fat diet-streptozotocin-induced diabetic rats
IF: 5.100 -
Effects of a Rosmarinus officinalis L. Extract and Rosmarinic Acid in Improving Streptozotocin-Induced Aortic Tissue Damages in Rats
IF: 4.800Journal: Nutrients (2024)Reactivity:RatSample Type:heart tissue,aorta tissue -
Integrating untargeted and oxylipins-targeted metabolomics to reveal the anti-obesity and hypolipidemic mechanism of conjugated linoleic acid in high-fat diet rats
IF: 3.800Journal: Journal of Functional Foods (2024)Reactivity:RatSample Type:serum -
Effect of Vitamin D3 on Chemerin and Adiponectin Levels in Uterus of Polycystic Ovary Syndrome Rats
IF: 6.000 -
Anaplastic Lymphoma Kinase Receptor: Possible Involvement in Anorexia Nervosa
IF: 5.900 -
The Potential Chemopreventive Effect of Andrographis paniculata on 1,2-Dimethylhydrazine and High-Fat-Diet-Induced Colorectal Cancer in Sprague Dawley Rats
IF: 5.600Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID:36982300Reactivity:RatSample Type:blood -
Effects of high dietary chicken protein on obesity development of rats fed high-fat diets
IF: 5.600Journal: Journal of Functional Foods (2023)Reactivity:RatSample Type:serum -
The Anti-Obesity and Anti-Steatotic Effects of Chrysin in a Rat Model of Obesity Mediated through Modulating the Hepatic AMPK/mTOR/lipogenesis Pathways
IF: 4.600 -
The Mechanism of Kelulut Honey in Reversing Metabolic Changes in Rats Fed with High-Carbohydrate High-Fat Diet
IF: 4.600 -
Mirabegron alleviates acetic acid-induced colitis in rats: Role of adiponectin and GSTM1/GSH detoxification pathway
IF: 4.500