QuicKey Pro Rat E2(Estradiol) ELISA Kit
中文名称:QuicKey Pro-大鼠雌二醇(Estradiol)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 3.13-200 pg/mL
- 灵敏度: 1.17 pg/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测大鼠血清、血浆、唾液、尿液中E2浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠E2抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗E2单克隆抗体,样本(或标准品中)的E2与包被抗原E2竞争结合HRP标记的抗E2单抗上的结合位点,形成包被抗原E2-HRP标记的抗E2单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,E2浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中E2的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 1 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 3.13-200 pg/mL |
灵敏度 | 1.17 pg/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆、唾液、尿液 |
特异性 | 可检测样本中的大鼠E2,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 6个月 |
数据处理 |
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Melatonin increases superoxide dismutase 2 (SOD2) levels and improves rat ovarian graft function after transplantation
IF: 3.800Journal: Journal of Ovarian Research (2024)Reactivity:RatSample Type:blood -
Comparison and investigation on characteristics of polycystic ovary syndrome rat models induced by letrozole, testosterone propionate, and high-fat diets
IF: 3.700Journal: REPRODUCTIVE BIOMEDICINE ONLINE (2024)Reactivity:RatSample Type:Serum -
Network pharmacology combined with lipidomics to reveal the regulatory effects and mechanisms of Kangzao granules in the hypothalamus of rats with central precocious puberty
IF: 3.400Journal: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2024)PMID:38422672Reactivity:RatSample Type:serum -
17β-Estradiol protects female rats from bilateral oophorectomy-induced nonalcoholic fatty liver disease induced by improving linoleic acid metabolism alteration and gut microbiota disturbance
IF: 3.400 -
Bisphenol A-induced polycystic ovary syndrome (PCOS) with hormonal and metabolic implications in rats
IF: 3.300Journal: REPRODUCTIVE TOXICOLOGY (2024)Reactivity:RatSample Type:serum -
Obesity aggravates neuroinflammatory and neurodegenerative effects of bisphenol A in female rats
IF: 2.800Journal: TOXICOLOGY MECHANISMS AND METHODS (2024)Reactivity:RatSample Type:Serum -
Moxibustion ameliorates ovarian function in premature ovarian insufficiency rats by activating cAMP/PKA/CREB to promote steroidogenesis in ovarian granulosa cells
IF: 2.700Journal: JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (2024)Reactivity:RatSample Type:Serum -
Hydroxycitric acid and capsaicin combination alleviates obesity-induced testicular apoptosis, oxidative stress and inflammation
IF: 2.400Journal: Systems Biology in Reproductive Medicine (2024)PMID:38323592Reactivity:RatSample Type:serum -
Dehydroepiandrosterone with a high-fat diet treatment at inducing polycystic ovary syndrome in rat model
IF: 2.100 -
Pancreastatin inhibitor PSTi8 improves ovarian health in Letrozole-HFD induced PCOS rats by ameliorating metabolic and reproductive parameters
IF: 2.100