QuicKey Pro Rat E2(Estradiol) ELISA Kit
中文名称：QuicKey Pro-大鼠雌二醇（Estradiol）酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.17 pg/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测大鼠血清、血浆、唾液、尿液中E2浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠E2抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗E2单克隆抗体，样本（或标准品中）的E2与包被抗原E2竞争结合HRP标记的抗E2单抗上的结合位点，形成包被抗原E2-HRP标记的抗E2单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，E2浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中E2的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.17 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆、唾液、尿液 |
| 特异性 | 可检测样本中的大鼠E2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
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The in vitro mechanism of Vaspinregulates the proliferation and steroidogenesis of rat lutein cells
IF: 2.000 -
Ameliorative effects of Myrtus communis L. extract involving the inhibition of oxidative stress on high fat diet-induced testis damage in rats
IF: 1.600Journal： BIOTECHNIC & HISTOCHEMISTRY (2024)Reactivity：RatSample Type：serum -
Cold exposure induces ovarian dysfunction through endoplasmic reticulum stress-mediated apoptosis
IF: 0.800 -
17β-oestradiol inhibits ferroptosis in the hippocampus by upregulating DHODH and further improves memory decline after ovariectomy
IF: 11.400 -
17β-Estradiol (E2) Upregulates the ERα/SIRT1/PGC-1α Signaling Pathway and Protects Mitochondrial Function to Prevent Bilateral Oophorectomy (OVX)-Induced Nonalcoholic Fatty Liver Disease (NAFLD)
IF: 7.000 -
High dietary copper intake induces perturbations in the gut microbiota and affects host ovarian follicle development
IF: 6.800Journal： ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023)PMID：36948015Reactivity：RatSample Type：Serum -
Reproductive and metabolic toxic effects of polystyrene microplastics in adult female Wistar rats: a mechanistic study
IF: 5.800Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023)PMID：36961641Reactivity：RatSample Type：Blood -
The Effects of Chronic Asprosin Administration on Sense of Smell and Sexual Behavior in Female Rats
IF: 4.100 -
Bushen Huoxue formula for the treatment of diminished ovarian reserve: A combined metabolomics and integrated network pharmacology analysis
IF: 4.000 -
Human umbilical cord mesenchymal stem cell-derived extracellular vesicles improve ovarian function in rats with primary ovarian insufficiency by carrying miR-145–5p
IF: 3.400
