QuicKey Pro Rat E2(Estradiol) ELISA Kit
中文名称:QuicKey Pro-大鼠雌二醇(Estradiol)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 3.13-200 pg/mL
- 灵敏度: 1.17 pg/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测大鼠血清、血浆、唾液、尿液中E2浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠E2抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗E2单克隆抗体,样本(或标准品中)的E2与包被抗原E2竞争结合HRP标记的抗E2单抗上的结合位点,形成包被抗原E2-HRP标记的抗E2单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,E2浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中E2的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 1 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 3.13-200 pg/mL |
灵敏度 | 1.17 pg/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆、唾液、尿液 |
特异性 | 可检测样本中的大鼠E2,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 6个月 |
数据处理 |
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1064nm Nd:YAG laser promotes chondrocytes regeneration and cartilage reshaping by upregulating local estrogen levels
IF: 2.800Journal: Journal of Biophotonics (2023)PMID:38041518Reactivity:RabbitSample Type:cartilage tissue -
Butylated starch alleviates polycystic ovary syndrome by stimulating the secretion of peptide tyrosine-tyrosine and regulating faecal microbiota
IF: 10.723 -
Moxibustion mitigates mitochondrial dysfunction and NLRP3 inflammatory activation in cyclophosphamide-induced premature ovarian insufficiency rats
IF: 6.780 -
Arsenic Induces Differential Neurotoxicity in Male, Female, and E2-Deficient Females: Comparative Effects on Hippocampal Neurons and Cognition in Adult Rats
IF: 5.682 -
Integrative transcriptomics and proteomics analyses to reveal the therapeutic effect and mechanism of Buxue Yimu Pills in medical-induced incomplete abortion rats
IF: 5.195Journal: JOURNAL OF ETHNOPHARMACOLOGY (2022)Reactivity:RatSample Type:serum -
Male reproductive system and simulated high-altitude environment: preliminary results in rats
IF: 3.054 -
Capsaicin prevents radiotherapy-induced premature ovarian failure in rats
IF: 1.973Journal: REPRODUCTION FERTILITY AND DEVELOPMENT (2022)DOI:10.1071/RD21235PMID:35101163Reactivity:RatSample Type:serum -
Behavioral and hormonal effects of prenatal and maternal separation stresses in postpartum rats
IF: 1.708 -
Apios americana Medikus: A novel and promising food for postpartum uterine involution
-
The effect of controlled ovarian hyperstimulation on ovarian reserve via PTEN pathway