QuicKey Pro Rat E2(Estradiol) ELISA Kit
中文名称：QuicKey Pro-大鼠雌二醇（Estradiol）酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 3.13-200 pg/mL
- 灵敏度： 1.17 pg/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测大鼠血清、血浆、唾液、尿液中E2浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠E2抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗E2单克隆抗体，样本（或标准品中）的E2与包被抗原E2竞争结合HRP标记的抗E2单抗上的结合位点，形成包被抗原E2-HRP标记的抗E2单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，E2浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中E2的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 3.13-200 pg/mL |
| 灵敏度 | 1.17 pg/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆、唾液、尿液 |
| 特异性 | 可检测样本中的大鼠E2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
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The Physicochemical Characteristics of Gelam Honey and Its Outcome on the Female Reproductive Tissue of Sprague–Dawley Rats: A Preliminary Study
IF: 4.412 -
CXCL12 and its receptors regulate granulosa cell apoptosis in PCOS rats and human KGN tumor cells
IF: 3.906Journal： REPRODUCTION (2021)PMID：33258800Reactivity：RatSample Type：Serum,Cell culture supernatant(ovarian granulosa cells) -
Moxibustion against Cyclophosphamide-Induced Premature Ovarian Failure in Rats through Inhibiting NLRP3-/Caspase-1-/GSDMD-Dependent Pyroptosis
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：33613687Reactivity：RatSample Type：Serum -
Intermittent fasting ameliorates di-(2-ethylhexyl) phthalate-induced precocious puberty in female rats: A study of the hypothalamic–pituitary–gonadal axis
IF: 2.376 -
Effects of exposure to a “safe” dose of bisphenol A on male reproductive function and the paternal contribution to the hypothalamic transcriptome profile
IF: 5.778 -
The Modulatory Role of Vitamin C in Boldenone Undecylenate Induced Testicular Oxidative Damage and Androgen Receptor Dysregulation in Adult Male Rats
IF: 5.014 -
Improvement in post-partum uterine involution in rats treated with Apios americana
IF: 1.879Journal： Journal of Zhejiang University-SCIENCE B (2019)PMID：31168971Reactivity：RatSample Type：serum -
Oral hormonal therapy with ethinylestradiol–levonorgestrel improves insulin resistance, obesity, and glycogen synthase kinase-3 independent of circulating mineralocorticoid in estrogen-deficient rats
IF: 2.210Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018)PMID：29400073Reactivity：RatSample Type：plasma
