QuicKey Pro Rat Pg(Progesterone) ELISA Kit
中文名称:QuicKey Pro-大鼠孕激素/孕酮(Pg)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 7.01 pg/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测大鼠血清、血浆中Pg浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠Pg抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗Pg单克隆抗体,样本(或标准品中)的Pg与包被抗原Pg竞争结合HRP标记的抗Pg单抗上的结合位点,形成包被抗原Pg-HRP标记的抗Pg单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Pg浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中Pg的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 1 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 7.01 pg/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的大鼠Pg,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 6个月 |
数据处理 |
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Effects of intracerebroventricular and intravenous administration of Kisspeptin-54 and Gonadotropin-releasing hormone agonist in rats with ovarian hyperstimulation
IF: 3.152 -
The effect of progesterone administration on the expression of metastasis tumor antigens (MTA1 and MTA3) in placentas of normal and dexamethasone-treated rats
IF: 2.742 -
Shoutai Wan Improves Embryo Survival by Regulating Aerobic Glycolysis of Trophoblast Cells in a Mouse Model of Recurrent Spontaneous Abortion
IF: 2.650Journal: Evidence-based Complementary and Alternative Medicine (2022)PMID:36212974Reactivity:RatSample Type:serum -
Behavioral and hormonal effects of prenatal and maternal separation stresses in postpartum rats
IF: 1.708 -
Apios americana Medikus: A novel and promising food for postpartum uterine involution
-
Development and Evaluation of Curcumin Encapsulated Self-assembled Nanoparticles as Potential Remedial Treatment for PCOS in a Female Rat Model
IF: 6.400Journal: International Journal of Nanomedicine (2021)PMID:34531655Reactivity:RatSample Type:serum -
Progesterone partially recovers placental glucose transporters in dexamethasone-induced intrauterine growth restriction
IF: 3.828 -
Chemical Fingerprint Analysis and Ultra-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolomics Study of the Protective Effect of Buxue Yimu Granule in Medical-Induced Incomplete Abortion Rats
IF: 4.225 -
Cadmium exposure during prenatal development causes testosterone disruption in multigeneration via SF-1 signaling in rats
IF: 3.775 -
Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells
IF: 1.593