QuicKey Pro Rat T(Testosterone) ELISA Kit
中文名称:QuicKey Pro-大鼠睾酮(Testosterone)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.07 ng/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测大鼠血清、血浆中T浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠T抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗T单克隆抗体,样本(或标准品中)的T与包被抗原T竞争结合HRP标记的抗T单抗上的结合位点,形成包被抗原T-HRP标记的抗T单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,T浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中T的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 1 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.07 ng/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的大鼠T,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 6个月 |
数据处理 |
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Q1:我想测细胞培养上清里睾酮的含量,含酚红培养基会对睾酮检测有影响么?
含酚红的培养基可能对睾酮检测有影响。酚红在培养基中被用来作为PH值的指示剂:中性时为红色,酸性时为黄色,碱性时为紫色。研究表明,酚红可以模拟固醇类激素的作用(特别是雌激素)。为避免固醇类反应及对细胞的毒性作用,培养细胞,尤其是哺乳类细胞时,用不加酚红的培养基。
介绍一个小窍门:培养基在分装时,留一管加酚红,放到培养箱内平衡(可以看出PH值的变化,又可以看培养基是否污染)。在培养前放到培养箱内平衡再使用(可以避免培养时使用酚红)。