QuicKey Pro Rat T(Testosterone) ELISA Kit
中文名称:QuicKey Pro-大鼠睾酮(Testosterone)酶联免疫吸附测定试剂盒
别称:

Price:
Size:
96 T48 T96 T × 5
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.07 ng/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测大鼠血清、血浆中T浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠T抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗T单克隆抗体,样本(或标准品中)的T与包被抗原T竞争结合HRP标记的抗T单抗上的结合位点,形成包被抗原T-HRP标记的抗T单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,T浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中T的浓度。 |
反应类型 | Competitive-ELISA |
规格 | 96 T/ 48 T/ 96 T × 5 |
反应时间 | 1 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.07 ng/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的大鼠T,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 6个月 |
数据处理 |
Zinc and selenium attenuate quaternary heavy metal mixture-induced testicular damage via amplification of the antioxidant system, reduction in metal accumulation, inflammatory and apoptotic biomarkers
IF: 2.300Antioxidant, anti-inflammatory, and anti-reprotoxic effects of kaempferol and vitamin E on lead acetate-induced testicular toxicity in male rats
IF: 1.200Journal: Open Veterinary Journal (2023)Reactivity:RatSample Type:serumLa exposición Posnatal a la Progesterona Exógena Exacerba la Producción y Función Anormales de Espermatozoides Inducidas Prenatalmente en Ratas
IF: 0.500Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats
IF: 7.675Curcumin Inhibits Hyperandrogen-Induced IRE1α-XBP1 Pathway Activation by Activating the PI3K/AKT Signaling in Ovarian Granulosa Cells of PCOS Model Rats
IF: 7.310Journal: Oxidative Medicine and Cellular Longevity (2022)PMID:36062194Reactivity:RatSample Type:serumAlleviating effects of selenium on fluoride-induced testosterone synthesis disorder and reproduction toxicity in rats
IF: 7.129Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022)PMID:36323150Reactivity:RatSample Type:testisStudies on testicular ultrastructural and hormonal changes in type-2 diabetic rats treated with highly active antiretroviral therapy conjugated silver nanoparticles
IF: 6.780Lactiplantibacillus plantarum CCFM1019 attenuate polycystic ovary syndrome through butyrate dependent gut–brain mechanism
IF: 6.317Proteomic Study on the Reproductive Toxicity of Tripterygium Glycosides in Rats
IF: 5.988Andrographolide exerted anti-inflammatory effects thereby reducing sex hormone synthesis in LPS-induced female rats, but had no effect on hormone production in healthy ones
IF: 5.988