QuicKey Pro Rat T(Testosterone) ELISA Kit
中文名称:QuicKey Pro-大鼠睾酮(Testosterone)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 0.16-10 ng/mL
- 灵敏度: 0.07 ng/mL
系列介绍 | 与传统ELISA试剂盒相比,将实验时间缩短了至少1-2小时,同时提高了试剂盒的灵敏度,让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术,旨在帮助客户以更为高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测大鼠血清、血浆中T浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用大鼠T抗原包被于酶标板上,实验时加入样品(或标准品)和辣根过氧化物酶(HRP)标记的抗T单克隆抗体,样本(或标准品中)的T与包被抗原T竞争结合HRP标记的抗T单抗上的结合位点,形成包被抗原T-HRP标记的抗T单抗复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,T浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中T的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 1 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;竞争法 |
检测范围 | 0.16-10 ng/mL |
灵敏度 | 0.07 ng/mL |
样本体积 | 50 μL |
样本类型 | 血清、血浆 |
特异性 | 可检测样本中的大鼠T,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 6个月 |
数据处理 |
-
Cysteamine mitigates torsion/detorsion-induced reperfusion injury via inhibition of apoptosis, oxidative stress and inflammatory responses in experimental rat model
IF: 2.775 -
Chlorogenic acid co-administration abates tamoxifen-mediated reproductive toxicities in male rats: An experimental approach
IF: 2.720 -
GPR120 agonist ameliorated insulin resistance and improved ovarian function
IF: 1.442 -
Treatment with acetate during late pregnancy protects dams against testosterone-induced renal dysfunction
-
Effects of exposure to a “safe” dose of bisphenol A on male reproductive function and the paternal contribution to the hypothalamic transcriptome profile
IF: 5.778Journal: CHEMOSPHERE (2020)Reactivity:RatSample Type:testis tissue,serum -
Suppression of p66Shc prevents hyperandrogenism-induced ovarian oxidative stress and fibrosis
IF: 4.124 -
Protocatechuic acid modulates reproductive dysfunction linked to furan exposure in rats
IF: 4.099 -
Synergic effects of aerobic exercise and eugenol supplement on germ cell development and testicular tissue structure in chlorpyrifos-treated animal model
IF: 3.056Journal: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020)PMID:32152857Reactivity:RatSample Type:serum -
Kolaviron suppresses dysfunctional reproductive axis associated with multi-walled carbon nanotubes exposure in male rats
IF: 3.056Journal: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020)PMID:32812151Reactivity:RatSample Type:Serum,Tissue homogenate -
Spironolactone reversed hepato-ovarian triglyceride accumulation caused by letrozole-induced polycystic ovarian syndrome: tissue uric acid—a familiar foe
IF: 2.050Journal: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020)PMID:31925474Reactivity:RatSample Type:plasma