QuicKey Pro Rat T(Testosterone) ELISA Kit
中文名称：QuicKey Pro-大鼠睾酮(Testosterone)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.07 ng/mL
| 系列介绍 | 与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。 |
| 用途 | 试剂盒用于体外定量检测大鼠血清、血浆中T浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用竞争ELISA法。用大鼠T抗原包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗T单克隆抗体，样本（或标准品中）的T与包被抗原T竞争结合HRP标记的抗T单抗上的结合位点，形成包被抗原T-HRP标记的抗T单抗复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，T浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中T的浓度。 |
| 反应类型 | Competitive-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 1 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;竞争法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.07 ng/mL |
| 样本体积 | 50 μL |
| 样本类型 | 血清、血浆 |
| 特异性 | 可检测样本中的大鼠T，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 6个月 |
| 数据处理 |
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Selenium attenuates diclofenac-induced testicular and epididymal toxicity in rats
IF: 1.951 -
Black soybean tempeh and purple sweet potato improve sperm quality in streptozotocin-induced diabetic rats
-
The role of gonadal hormones in the hypoglossal discharge activity of rats exposed to chronic intermittent hypoxia
IF: 3.103 -
Candesartan in a rat model of testicular toxicity: New insight on its protective mechanism
IF: 3.005 -
Attenuation potentials of royal jelly against hydroxyurea-induced infertility through inhibiting oxidation and release of pro-inflammatory cytokines in male rats
IF: 2.914Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2019)PMID：31127524Reactivity：RatSample Type：serum -
Nano-selenium attenuates nickel-induced testosterone synthesis disturbance through inhibition of MAPK pathways in Sprague-Dawley rats
IF: 2.649 -
Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats
IF: 2.468Journal： International Journal of Environmental Research and Public Health (2019)PMID：30974849Reactivity：RatSample Type：serum -
Sodium acetate and androgen receptor blockade improve gestational androgen excess-induced deteriorated glucose homeostasis and antioxidant defenses in rats: roles of adenosine deaminase and xanthine oxidase activities
IF: 4.414 -
Intracerebroventricular injection of histamine induces the hypothalamic-pituitary-gonadal axis activation in male rats
IF: 3.125 -
Grape seed procyanidins extract attenuates Cisplatin-induced oxidative stress and testosterone synthase inhibition in rat testes
IF: 1.582Journal： Systems Biology in Reproductive Medicine (2018)PMID：29613814Reactivity：RatSample Type：serum
