Rat Bcl-2(B-cell Lymphoma/Leukemia 2) ELISA Kit
中文名称：大鼠B细胞淋巴瘤因子2(Bcl-2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠Bcl-2抗体包被于酶标板上，实验时样品（或标准品）中的大鼠Bcl-2会与包被抗体结合。后依次加入生物素化的抗大鼠Bcl-2抗体和辣根过氧化物酶标记的亲和素，抗大鼠Bcl-2抗体与结合在包被抗体上的大鼠Bcl-2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Bcl-2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Bcl-2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠Bcl-2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Malva parviflora seed oil; Isolation, gas chromatographic profiling and its cardioprotective activity against myocardial infraction in animal model
IF: 3.8Journal： JOURNAL OF KING SAUD UNIVERSITY SCIENCE (2023)Reactivity：RatSample Type：plasma -
Pentoxifylline as Add-On Treatment to Donepezil in Copper Sulphate-Induced Alzheimer’s Disease-Like Neurodegeneration in Rats
IF: 3.7 -
Vitamin D alleviates cognitive dysfunction and brain damage induced by copper sulfate intake in experimental rats: focus on its combination with donepezil
IF: 3.600Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023)PMID：36864348Reactivity：RatSample Type：hippocampal -
Sodium acetate ameliorates doxorubicin-induced cardiac injury via upregulation of Nrf2/HO-1 signaling and downregulation of NFkB-mediated apoptotic signaling in Wistar rats
IF: 3.6Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023)PMID：37458777Reactivity：RatSample Type：cardiac tissue -
Acetyl-11-Keto-Beta Boswellic Acid(AKBA) modulates CSTC-pathway by activating SIRT-1/Nrf2-HO-1 signalling in experimental rat model of obsessive-compulsive disorder: Evidenced by CSF, blood plasma and histopathological alterations
IF: 3.4 -
Neuroprotection by trans-resveratrol in rats with N-methyl-D-aspartate (NMDA)–induced retinal injury: Insights into the role of adenosine A1 receptors
IF: 2.900 -
Esculeoside A Decreases Diabetic Cardiomyopathy in Streptozotocin-Treated Rats by Attenuating Oxidative Stress, Inflammation, Fibrosis, and Apoptosis: Impressive Role of Nrf2
IF: 2.6 -
Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosis
IF: 1.7Journal： Asian Pacific Journal of Tropical Biomedicine (2023)Reactivity：RatSample Type：heart -
Irbesartan reprofiling for the amelioration of ethanol-induced gastric mucosal injury in rats: Role of inflammation, apoptosis, and autophagy
IF: 6.780 -
Lycopene Modulates Oxidative Stress and Inflammation in Hypercholesterolemic Rats
IF: 5.215
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
