Rat NFKB-p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
中文名称：大鼠核因子κB亚基p105(NFKB-p105)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 0.16-10 ng/mL
- 灵敏度： 0.1 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠NFKB-p105抗体包被于酶标板上，实验时样品（或标准品）中的大鼠NFKB-p105会与包被抗体结合。后依次加入生物素化的抗大鼠NFKB-p105抗体和辣根过氧化物酶标记的亲和素，抗大鼠NFKB-p105抗体与结合在包被抗体上的大鼠NFKB-p105结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NFKB-p105浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NFKB-p105的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.16-10 ng/mL |
| 灵敏度 | 0.1 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠NFKB-p105，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
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In vivo exposure to bisphenol F induces oxidative testicular toxicity: role of Erβ and p53/Bcl-2 signaling pathway
Journal： Frontiers in Reproductive Health (2023)Reactivity：RatSample Type：testicular tissue -
Glutamine restores testicular glutathione-dependent antioxidant defense and upregulates NO/cGMP signaling in sleep deprivation-induced reproductive dysfunction in rats
IF: 7.419Journal： BIOMEDICINE & PHARMACOTHERAPY (2022)PMID：35247715Reactivity：RatSample Type：Testicular ,epididymal -
Banana peel ameliorated hepato-renal damage and exerted anti-inflammatory and anti-apoptotic effects in metal mixture mediated hepatic nephropathy by activation of Nrf2/ Hmox-1 and inhibition of Nfkb pathway
IF: 5.572 -
Zinc normalizes hepatic lipid handling via modulation of ADA/XO/UA pathway and caspase 3 signaling in highly active antiretroviral therapy-treated Wistar rats
IF: 5.168Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2022)Reactivity：RatSample Type：plasma,hepatic tissues -
Chondroitin and glucosamine sulphate reduced proinflammatory molecules in the DRG and improved axonal function of injured sciatic nerve of rats
IF: 4.996 -
Zinc and selenium mitigated heavy metals mixture (Pb, Al, Hg and Mn) mediated hepatic-nephropathy via modulation of oxido-inflammatory status and NF?kB signaling in female albino rats
IF: 4.571 -
Tetramethylpyrazine Attenuates Cognitive Impairment Via Suppressing Oxidative Stress, Neuroinflammation, and Apoptosis in Type 2 Diabetic Rats
IF: 4.414 -
Acetate restores hypothalamic-adipose kisspeptin status in a rat model of PCOS by suppression of NLRP3 immunoreactivity
IF: 3.925 -
Ameliorative effect of carveol on scopolamine-induced memory impairment in rats
IF: 2.532Journal： Iranian Journal of Basic Medical Sciences (2022)Reactivity：RatSample Type：brain tissues -
Naringenin Prevents Inflammation, Apoptosis, and DNA Damage in Potassium Oxonate-Induced Hyperuricemia in Rat Liver Tissue: Roles of Cytochrome C, NF-κB, Caspase-3, and 8-Hydroxydeoxyguanosine
IF: 2.363Journal： Metabolic Syndrome and Related Disorders (2022)PMID：35796694Reactivity：RatSample Type：Liver tissue
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