Rat NFKB-p65(Nuclear Factor Kappa B p65) ELISA Kit
中文名称：大鼠核因子κB亚基p65(NFKB-p65)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 78.13-5000 pg/mL
- 灵敏度： 46.88 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠NFKB-p65抗体包被于酶标板上，实验时样品（或标准品）中的大鼠NFKB-p65会与包被抗体结合。后依次加入生物素化的抗大鼠NFKB-p65抗体和辣根过氧化物酶标记的亲和素，抗大鼠NFKB-p65抗体与结合在包被抗体上的大鼠NFKB-p65结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，NFKB-p65浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中NFKB-p65的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 78.13-5000 pg/mL |
| 灵敏度 | 46.88 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠NFKB-p65，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Zn and Se abrogate heavy metal mixture induced ovarian and thyroid oxido-inflammatory effects mediated by activation of NRF2-HMOX-1 in female albino rats
Journal： Current Research in Toxicology (2022)PMID：36624872Reactivity：RatSample Type：ovarian,thyroid -
Pharmacological evaluation of continentalic acid for antidiabetic potential
IF: 6.530 -
Ginkgo biloba extract (EGb 761) mitigates methotrexate-induced testicular insult in rats: Targeting oxidative stress, energy deficit and spermatogenesis
IF: 6.530Journal： BIOMEDICINE & PHARMACOTHERAPY (2021)PMID：34560547Reactivity：RatSample Type：testicular tissue -
Dry heated sorghum BRS 305 hybrid flour as a source of resistant starch and tannins improves inflammation and oxidative stress in Wistar rats fed with a high-fat high-fructose diet
IF: 5.396 -
Targeting inflammation, autophagy, and apoptosis by troxerutin attenuates methotrexate-induced renal injury in rats
IF: 4.932Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2021)PMID：34953450Reactivity：RatSample Type：renal tissue -
Effects of catechin on a rodent model of autism spectrum disorder: implications for the role of nitric oxide in neuroinflammatory pathway
IF: 4.530 -
Activation of the Nrf2/HO-1 signaling pathway by dimethyl fumarate ameliorates complete Freund's adjuvant-induced arthritis in rats
IF: 4.432Journal： EUROPEAN JOURNAL OF PHARMACOLOGY (2021)PMID：33745959Reactivity：RatSample Type：Tissue homogenate(Joint Tissue) -
Bergapten Attenuates Nitroglycerin-Induced Migraine Headaches through Inhibition of Oxidative Stress and Inflammatory Mediators
IF: 4.418 -
Germinated millet flour (Pennisetum glaucum (L.) R. Br.) reduces inflammation, oxidative stress, and liver steatosis in rats fed with high-fat high-fructose diet
IF: 3.616Journal： JOURNAL OF CEREAL SCIENCE (2021)Reactivity：ratSample Type：Blood -
Neuroprotective effect of green and roasted coffee bean extracts on cerebral ischemia-induced injury in rats
IF: 3.584
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
