Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

中文名称：大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒

货号：E-EL-R2856
文献（863）
说明书

说明书

别称：

Tnf、Tnfa、Tnfsf2

Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

Price: ¥2400.00 ¥1650.00 ¥10200.00

Size:

96 T 48 T 96 T × 5

反应性： Rat
检测范围： 15.63-1000 pg/mL
灵敏度： 9.38 pg/mL

产品应用	ELISA
检测原理	本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。
反应类型	Sandwich-ELISA
规格	96 T / 48 T / 96 T × 5
反应时间	3 h 30 min
反应性	Rat
检测方法	比色法;ELISA;夹心法
检测范围	15.63-1000 pg/mL
灵敏度	9.38 pg/mL
样本体积	100 μL
样本类型	血清、血浆或其他生物体液
特异性	可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应
精密度	板内，板间变异系数均<10%
回收率	80%-120%
储存条件	2-8℃
有效期	12个月
操作步骤
数据处理	ELISA结果计算

Melatonin mitigated methotrexate-induced hepatotoxicity through interrelated biological processes

IF: 2.6

Journal： MOLECULAR BIOLOGY REPORTS (2024)

DOI：10.1007/s11033-024-09792-z

Reactivity：Rat

Sample Type：serum
Combination Therapy with Vitamin D and Metformin: A Potential Approach to Mitigate Testicular Dysfunction in Type 2 Diabetes Mellitus

IF: 2.6

Journal： Reproductive Sciences (2024)

DOI：10.1007/s43032-024-01708-3

Reactivity：Rat

Sample Type：Testicular tissue
Preventive effects of melatonin and amifostine on irradiated rats with experimental periodontitis

IF: 2.6

Journal： BMC Oral Health (2024)

DOI：10.1186/s12903-024-05251-0

Reactivity：Rat

Sample Type：serum
Autophagy-mediated ferroptosis is involved in development of severe acute pancreatitis

IF: 2.5

Journal： BMC GASTROENTEROLOGY (2024)

DOI：10.1186/s12876-024-03345-1

Reactivity：Rat

Sample Type：Serum
Effect of madecassic acid on retinal oxidative stress, inflammation and Growth Factors in streptozotocin-induced diabetic rats

IF: 2.5

Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2024)

DOI：10.1016/j.bbrc.2024.150745

Reactivity：Rat

Sample Type：retina tissue
Polygala tenuifolia root extract attenuates ischemic stroke by inhibiting HMGB1 trigger neuroinflammation

IF: 2.5

Journal： FITOTERAPIA (2024)

DOI：10.1016/j.fitote.2024.106280

Reactivity：Rat

Sample Type：serum
Pharmacokinetics of seven major components in Aβ1-42-treated rats after oral administration of an aqueous extract of Curculiginis Rhizoma and Epimedii Folium

IF: 2.5

Journal： Journal of Natural Medicines (2024)

DOI：10.1007/s11418-024-01861-8

Reactivity：Rat

Sample Type：hippocampus tissue,serum
Prevention of colon enlargement by TNF-α antagonist in a streptozotocin-induced diabetic rat model

IF: 2.5

Journal： HISTOLOGY AND HISTOPATHOLOGY (2024)

DOI：10.14670/HH-18-735

Reactivity：Rat

Sample Type：plasma
Effects of mid?gestational sevoflurane and magnesium sulfate on maternal oxidative stress, inflammation and fetal brain histopathology

IF: 2.4

Journal： Experimental and Therapeutic Medicine (2024)

DOI：10.3892/etm.2024.12574

Reactivity：Rat

Sample Type：serum
Hyperbaric oxygen preconditioning rescues prolonged underwater exercise-induced hippocampal dysfunction by regulating microglia activation and polarization

IF: 2.4

Journal： NEUROSCIENCE RESEARCH (2024)

DOI：10.1016/j.neures.2024.05.004

Reactivity：Rat

Sample Type：hippocampal tissue

Species

{{relatedElisaPage.numTotal}} Results

All

Canine

Human

Mouse

Porcine

Rat

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual

Cat.No.：{{item.cat}}

{{index}}{{goods_show_value}}