Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称：大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
-
Pandanus amaryllifoius Roxb. Leaves Ethanol Extract Ameliorates Lipid and Proinflammatory Cytokines Profiles in a Rat Model of Dyslipidemia
IF: 1.2Journal： Journal of Pharmacopuncture (2024)Reactivity：RatSample Type：serum -
Isoflurane preconditioning protects against renal ischemia/reperfusion injury in diabetes via activation of the Brg1/Nrf2/HO-1 signaling pathway
IF: 1.1Journal： Acta Cirurgica Brasileira (2024)Reactivity：RatSample Type：renal tissue -
Therapeutic potential of Tithonia diversifolia extract: Modulating IL-35, TNF-α, and hematology profile in streptozotocin-induced rat model
IF: 0.9Journal： Open Veterinary Journal (2024)Reactivity：RatSample Type：serum -
Pinobanksin Attenuates Cerebral Ischemia–Reperfusion Injury in Rats Through Mitigating the Inflammatory Responses
IF: 0.6Journal： Pharmacognosy Magazine (2024)Reactivity：RatSample Type：Serum,Brain Tissue -
Monotropein Reduces Retinal Inflammation, Oxidative Stress, and Dyslipidemia in Streptozotocin-induced Diabetic Retinopathy in Rats
IF: 0.6Journal： Pharmacognosy Magazine (2024)Reactivity：RatSample Type：serum -
Accelerating Healing of Excisional Wound with a Hydrogel Containing Hyacinth Bean (Dolichos lablab) Extract in a Rat Model
IF: 0.3Journal： Current Drug Therapy (2024)Reactivity：RatSample Type：skin tissue -
Tocilizumab Reduces Lung Injury in a Rat Lung Ischemia and Reperfusion Model
Journal： Thoracic Research and Practice (2024)Reactivity：RatSample Type：serum -
Cardiac-targeted delivery of nuclear receptor RORα via ultrasound targeted microbubble destruction optimizes the benefits of regular dose of melatonin on sepsis-induced cardiomyopathy
IF: 11.300 -
Downregulation of ATF-4 Attenuates the Endoplasmic Reticulum Stress–Mediated Neuroinflammation and Cognitive Impairment in Experimentally Induced Alzheimer's Disease Model
IF: 5.1Journal： MOLECULAR NEUROBIOLOGY (2023)Reactivity：RatSample Type：hippocampus (HP) tissue,cerebral cortex tissue -
Atorvastatin Promotes Pro/anti-inflammatory Phenotypic Transformation of Microglia via Wnt/β-catenin Pathway in Hypoxic-Ischemic Neonatal Rats
IF: 5.1
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
