Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit
中文名称：大鼠肿瘤坏死因子α(TNF-α)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的大鼠TNF-α会与包被抗体结合。后依次加入生物素化的抗大鼠TNF-α抗体和辣根过氧化物酶标记的亲和素，抗大鼠TNF-α抗体与结合在包被抗体上的大鼠TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，TNF-α浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中TNF-α的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠TNF-α，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 操作步骤 | |
| 数据处理 |
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HMGB1/RAGE pro-inflammatory axis promotes vascular endothelial cell apoptosis in limb ischemia/reperfusion injury
IF: 3.743 -
Baicalein administered in the subacute phase ameliorates ischemia-reperfusion-induced brain injury by reducing neuroinflammation and neuronal damage
IF: 3.743 -
Myocardial caspase-3 and NF-κB activation promotes calpain-induced septic apoptosis: The role of Akt/eNOS/NO pathway
IF: 3.448 -
Tao-Hong-Si-Wu decoction reduces ischemia reperfusion rat myoblast cells calcium overloading and inflammation through the Wnt/IP3R/CAMKII pathway
IF: 3.448Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2019)PMID：30950126Reactivity：RatSample Type：Cell culture supernate -
Evodiamine alleviates kidney ischemia reperfusion injury in rats: A biochemical and histopathological study
IF: 3.448Journal： JOURNAL OF CELLULAR BIOCHEMISTRY (2019)PMID：31099131Reactivity：RatSample Type：Tissue homogenate -
Inhibition of JAK-STAT and NF-κB signalling systems could be a novel therapeutic target against insulin resistance and type 2 diabetes
IF: 3.448 -
Preventive effects of fraxin on ischemia/reperfusion-induced acute kidney injury in rats
IF: 3.448 -
Antidiabetic property of miracle fruit plant (Synsepalum dulcificum Shumach. & Thonn. Daniell) leaf extracts in fructose-fed streptozotocin-injected rats via anti-inflammatory activity and inhibition of carbohydrate metabolizing enzymes
IF: 3.414 -
TLR2 and TLR4 mediate an activation of adipose tissue renin-angiotensin system induced by uric acid
IF: 3.362 -
Apigenin attenuates acrylonitrile-induced neuro-inflammation in rats: Involved of inactivation of the TLR4/NF-κB signaling pathway
IF: 3.361
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